WormBase Tree Display for RNAi: WBRNAi00085171

WBRNAi00085171 Homol: Homol_homol: F53G12:RNAi
      W04G5:RNAi
  Sequence_info: PCR_product: sjj_F53G12.1
  Experiment: Laboratory: HY
    Date: 20 Dec 2010 00:00:00
    Genotype: unc-119(ed3) [opt-2::GFP::pgp-1, unc-119(+)]
    Treatment: For determination of endocytosis after RNAi treatment, PGP-1::GFP animals were synchronized to the L1 stage by hypochlorite treatment and overnight starvation in M9 media at room temperature and then seeded onto RNAi plates and grown to the L4 stage at 20C, before being exposed to OP50-Cry5B for 2 hr at 20C. 1:10 dilutions of rab-11.1 RNAi were made by combining one part RNAi culture with nine parts pL4440 (empty vector) bacteria
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F53G12.1 Inferred_automatically: RNAi_primary
    Gene: WBGene00004274 Inferred_automatically: RNAi_primary
    Transcript: F53G12.1.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00038144
  Phenotype: WBPhenotype:0000679
    WBPhenotype:0001422 Remark: RNAi causes significant inhibition of Cry5B-induced endocytic response, as indicated by the PGP-1::GFP transgene
  Remark: Authors note that the rab-11.1 RNAi clone was a gift from Barth Grant. Ahringer clone used for curation. Authors also note that due to the sequence similarity with rab-11.2, this clone likely also knocks down levels of rab-11.2
  Method: RNAi