WormBase Tree Display for RNAi: WBRNAi00085451
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| WBRNAi00085451 | Homol | Homol_homol | C17E4:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | cenix:77-a7 | |||
| Experiment | Laboratory | OD | |||
| Date | 24 Mar 2011 00:00:00 | ||||
| Strain | WBStrain00029219 | ||||
| Treatment | Larval L4 stage worms were soaked in dsRNA for 24 hrs at 20C. After soaking, the worms were transferred to NGM plates seeded with OP50 E. coli and allowed to recover for 48 hrs prior to imaging. | ||||
| Temperature | 20 | ||||
| Delivered_by | Soaking | ||||
| Inhibits | Predicted_gene | C17E4.9a | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00007646 | Inferred_automatically | RNAi_primary | ||
| Transcript | C17E4.9b | Inferred_automatically | RNAi_primary | ||
| C17E4.9a.1 | Inferred_automatically | RNAi_primary | |||
| DB_info | Database | Phenobank2 | Gene&RNAID | GeneID=503808 | |
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00038381 | ||||
| Phenotype | WBPhenotype:0000186 | Remark | Oocyte budding delayed. In wildtype as as they round the turn in the gonad, oocytes bud off of the rachis tip, here there is a delay in this process. | ||
| WBPhenotype:0000688 | |||||
| WBPhenotype:0001944 | |||||
| WBPhenotype:0001945 | |||||
| WBPhenotype:0001951 | Remark | Pachytene extends to the turn in the gonad. | |||
| WBPhenotype:0001952 | Remark | Nuclear spacing disrupted prior to expansion in the germline. | |||
| Nuclei irregularly positioned in germline compartments. | |||||
| Nuclei irregularly positioned in proximal ooctyes. | |||||
| WBPhenotype:0001953 | Remark | Nuclei are mispositioned within compartments of the developing germline and/or have fallen out of these compartments into the rachis. | |||
| WBPhenotype:0001972 | |||||
| WBPhenotype:0001980 | Remark | Delayed compartment expansion at turn in gonad. | |||
| WBPhenotype:0001982 | Remark | PI4,5P2 accumulation in cytoplasm or nucleus of germ cells. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes. | |||
| Soluble PI4,5P2 in proximal oocytes. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes. | |||||
| Soluble PI4,5P2 throughout gonad. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes. | |||||
| Remark | Experiment SS135 | ||||
| Method | RNAi |
