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WormBase Tree Display for RNAi: WBRNAi00085755

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Name Class

WBRNAi00085755HomolHomol_homolY110A7A:RNAi
Sequence_infoDNA_textgaggagcgcctgaaagtgattgaaaaaattccacgtgtatttcccgatgtccaagtaactgttgcagactccaagaatggtttttgaattattcaatatttcattttaatgtttatattgcagataacgaggcggaaagttacgattttgaaaaagaatccgcagctggactccgagctactcaacgatccatctatcgatcagatcttcggaagcagttgctttcatctaataaacatagagcacaagacacatctggtaaaaattagtatccaatgattgttcaaaattcgtcaaattttatagaagatcaggaattcatgaaaaatgagcttgtcgaggaggagctagcaaatagtttggccactatggcgagatcattcaaaacgatgatgtcggctgctggagatgtgattaaagaagatacagaaagagcaatattaatggcgaaagaagtcgatgataataaaacagctctcggggtaatttcttcactatataaacttcaaataatagtcttaaaatttccagattcaaagtgagcgagttgagcgtcacgcctacaaatgtggttatgactgtttcaaggtcatgctcattgttctgatttttatgagcttcgtatcaatggttcttatgatgaaaatttt
ExperimentLaboratoryOD
Date24 Mar 2011 00:00:00
StrainWBStrain00029219
TreatmentLarval L4 stage worms were soaked in dsRNA for 24 hrs at 20C. After soaking, the worms were transferred to NGM plates seeded with OP50 E. coli and allowed to recover for 48 hrs prior to imaging.
Temperature20
Delivered_bySoaking
InhibitsPredicted_geneY110A7A.11Inferred_automaticallyRNAi_primary
GeneWBGene00022460Inferred_automaticallyRNAi_primary
TranscriptY110A7A.11.1Inferred_automaticallyRNAi_primary
DB_infoDatabasePhenobank2Gene&RNAIDGeneID=517870
SpeciesCaenorhabditis elegans
ReferenceWBPaper00038381
PhenotypeWBPhenotype:0000186RemarkOocyte budding delayed. In wildtype as as they round the turn in the gonad, oocytes bud off of the rachis tip, here there is a delay in this process.
WBPhenotype:0000688
WBPhenotype:0001810RemarkOocyte juctions are irregular.
WBPhenotype:0001969RemarkInternal compartments in rachis distal to turn in gonad.
WBPhenotype:0001982RemarkInternal PI4,5P2 debris in germ cells. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes.
PI4,5P2 accumulation in cytoplasm or nucleus of germ cells. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes.
RemarkExperiment SS444
MethodRNAi