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WormBase Tree Display for RNAi: WBRNAi00085862

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Name Class

WBRNAi00085862HomolHomol_homolC35D10:RNAi
Sequence_infoPCR_productWBPaper00038381:C35D10_1_1
ExperimentLaboratoryOD
Date24 Mar 2011 00:00:00
StrainWBStrain00029219
TreatmentLarval L4 stage worms were soaked in dsRNA for 24 hrs at 20C. After soaking, the worms were transferred to NGM plates seeded with OP50 E. coli and allowed to recover for 48 hrs prior to imaging.
Temperature20
Delivered_bySoaking
InhibitsPredicted_geneC35D10.13Inferred_automaticallyRNAi_primary
GeneWBGene00016449Inferred_automaticallyRNAi_primary
TranscriptC35D10.13.1Inferred_automaticallyRNAi_primary
DB_infoDatabasePhenobank2Gene&RNAIDGeneID=504678
SpeciesCaenorhabditis elegans
ReferenceWBPaper00038381
PhenotypeWBPhenotype:0000182RemarkApoptosis reduced in the germline.
WBPhenotype:0000186RemarkOocyte budding delayed. In wildtype as as they round the turn in the gonad, oocytes bud off of the rachis tip, here there is a delay in this process.
WBPhenotype:0000688
WBPhenotype:0001940RemarkRachis constriction in proximal region delayed.
WBPhenotype:0001944
WBPhenotype:0001950RemarkShortened diplotene region.
WBPhenotype:0001951RemarkPachytene extends to the turn in the gonad.
WBPhenotype:0001980RemarkDelayed compartment expansion at turn in gonad.
WBPhenotype:0001982RemarkInternal PI4,5P2 debris in germ cells. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes.
PI4,5P2 accumulation in cytoplasm or nucleus of germ cells. PI4,5P2 is phosphatidylinositol 4,5-bisphosphate which is normally a phospolipid component of cell membranes.
RemarkExperiment SS551
MethodRNAi