WormBase Tree Display for RNAi: WBRNAi00087587

WBRNAi00087587 Homol: Homol_homol: CHROMOSOME_I:RNAi
  Sequence_info: DNA_text: atgacatcatctcgacaaaaagatgatgagattgaccaattgagagccaagacagacttcctcaatccatcgatttcggcagattgggcgaaaaaaaagttgatgtgggtacccagtgagaaggatggattcgcacttggagctgtcatcggggaaccacatgcagacggaactatcgacattgagcttatggagactggcgaacggcagcgtgtttcgtctgatgattgccagaagccaaatcctccgaaatatgacaaatgcgaggatatgtcaatgctgacgtgtctgaatgaagcgtcggttcttcataatctaaagcaacgatatttctcgaatctctactacacatactctggcttgttctgcgttgtaataaatccatacaaacgaattccaatctacacggatacaattgctgaacagttcaaatgcaaaaagagaaaggagatgccaccacacatatttgctgttgccgatgaagcctaccgcagcatgcttcaagaacgagatgatcagtcaattctctgcactggagaatcgggagctggaaagactgaaaatacaaagaaagtgattcagtatttggcttatgtggcaaatcggtcgatggtgaagaatcggaagacttccgttgatctggacacatctacaaaccggatcatgggccagcttgaggaacagcttttgcaagcaaatcccatactcgaggctttcggaaacagtaagacagtgaagaacgataattccagtagatttggaaagttcattcgcgttcatttcgacagcacagggtgcatttctggagcgaatattgaattttatcttctggagaaatctcgcgtgctgaaacaagctcccaatgagcgatcgttccacattttctaccagttgctgaaaggactctcaaaagtacagagagaacattatctcctagaagacagcctatccaagtacaagtttgtctcaaatggtgacagtaaattggctggagttgatgatggagcagagatgaaggaaacactgaatgccatgtcaattatgggattgaacgacgaagagattggaggaatacttagagttgtctcggcagttatgctctttggaaatcttgaattttctcacgaaaacaagaataacgatcaggctgttctgctcaatgacgctgttgctcaaaaaatcgcttcacttctcggagtcaatgttaccgaattgatgcgtgcattcttgaagccaaaaatcaaggttcaaagggatttggtccatcgagctcaaagcgtagaccaagtcaatttctctgtgggagccattgcgaaggcctcgtatgagcgactcttcagatggttggttcatcgcctcaacaaatctctcgatcggacccgtcaacaatctgtcagctttatcggaattcttgatattgctggttttgaaatttttgaaacaaactcattcgagcagctatgtatcaactacactaacgagaaacttcaacaactcttcaacaatacaatgttcgttcgtgaacagcaagaatatcttgatgaaggattggagtggaagttcttggatttcggcctcaatcttcaaccgactattgacttgattgataagcctatgggaatcatgtcaactcttgatgatgtttgtctattcccacaaggaaatgatcaatcatttgttcaacgcctcaataacactcattctcaacatccgaagtacgtcgttccagagattcgctcacgaagcgattttgccgttgttcattatgcgggccgagtcgactatcaatctgaaggttggcgcgtgaaaaacatggatccattgaatgagaatgtgattgatgtgctcaaaacttcaaaggaatctcttattgtcgacatgtggaaagatatcgctgatgtttgcagtttgtcagctgctgacagtacttcagataccggagtatttggatctcgtgttccaaagaagggaatgttccgtactgtttctcaactttacaaagaacaattggctcgtcttatgagtactctcaataacaccaatcctcactttgttcgctgcatcattccaaatcacgaaaagaaacatggagttctcaacgctcatcttgttcttgatcaattaagatgcaacggagtgttggaaggaattcgtatatgccgtcaaggattccctacgcggctcccgttccaagaattccgtcaacgctacgagaaacttcttgcccctgatgtcaacccagctggattcatggacggtaaaaacgccgtttatcgtattgttcaatatctggaagttgacgccaacctcttccgtattggtcaatctaagatcttcttccgttctggagttatagctgagtttgaggagatgcgtgatcagaagctttccgctcttattgaatctttccaagctcaatgtcgtggatggctaggacgccgagtgatggtacgcagacgagaacaagaagttgctatcaaaattcttcaaagaaatggtttggcatggatgagactgcgtgaatggcaatggtggagacttctgaccaaagtgaagccacttctcgaagttaccaacaaagacgaattaattgctgagcgagaacaagaactgaaagtgacagctgaaaagcttcgtagatccgaggtattcatctccgattacaagcaacaaatggaaaaaatggacgaggagcggctggttttaaagacgagactcgatgctgaatcgtctgagagagctgaaatatttgaggaacgttcccgaatggctgctcggagagacgaacttgaaggaatccttgaagaagtttcgaagagactggagatcgaagagcaaaaggcaaagaaggctgattctgaatcccgaaaattgacggagatggttagacatctcgaagagaatcttgaagatgaagaaagaagccgtcagaaattgttgctcgaaaaaaattcaattgaatctcggttgaaggaactggaagcacaaggactcgagcttgaagattctggaaacaagttgtcgaaagaaaagaaggctttggaagaacgctgtgaagatctttcgtcacgtttaattgacgaggttgaacgatcgaaacaattggtcaaggcaaaggctcgcctggaagctacggttgcagagataaacgatgaattggaaaaagagaagcagcagagacataatgccgaaacagctcgtcgtgctgccgaaacacagctccgcgaggaacaagaatcatgcctggaaaagactcgtaaagcggaagagttgacaaatcagctgatgcgcaaagagtcggaattgtcgcaaataagcattcggaacgacgaagaactcgcagctcggcaacagttagaacgtgaaattcgggaaattcgtgcacagcttgacgatgcaattgaggagacaaacaaggagaaagctgctcgtcagaaagcagagaaagctcgtcgagatatggctgaagaattggaatcctacaagcaagaattggaagaatccaatgataaaaccgttctccactcgcaactcaaagctaaacgagatgaagagtatgctcatttgcagaaacaacttgaggaaacagtcaaatcttctgaagaagttgtggaggaaatgaaggctcagaatcagaaaaagattgaggagttgaatgagacgatcgatcagttgaaacgtcagaaaatttcggctgacaaggcaaaatcttctgctgaatctgataacgagaacttccgtgccgagctgtccaacatagcatcggctcgtctggaggcggaaaagaagcggaaagcggccgagacgtcgttgatggagaaggatcataagatgcgtgaaatgcagtcaaatcttgatgatcttatggctaagttatcgaagatgaacaacgagcttgaaagcattcaaaaagcaaagagtgctgacgaaactctgaattctaaccttctcaagaagaatgcttcactcgacatgcagttgtctgagcttactgaagcttctgaggaagatcgtcgaactagagccacgttaaacaataaaattcgtcagctcgaagaagatcttgcggttgctgttgaagccagagatgacgcacttgacgcacaagaaaagatcgaaaaagaggtcaaagaagtcaaatctctgttggccgaggctagaaagaagttggatgaggaaaatagagaggtgatggaagaattgcggaagaagaaggagaaggagttgtcagccgagaaagagcgggccgacatggctgaacaagcaagagataaggctgaacgggccaagaagaaggctatccaggaggctgaggacgtgcaaaaagagttgactgatgtggtggccgcaactagagagatggagagaaaaatgaggaagtttgatcaacagctcgccgaggagagaaacaacactttgctcgctcagcaagaaagagatatggctcatcaaatgctacgtgatgcagagacgaaggctctggtgttgtcaaatgaattatccgagaaaaaggatattgtggatcagttagaaaaggacaaacggactctcaaactagagattgacaatttggcctccaccaaggatgatgctggaaagaatgtttacgagttggagaagacgaaacgtcgccttgatgaagagttgagtcgtgcggaacaacaaatcattgaactagaagatgctctccaacttgctgatgatgctagaagcagagttgaagtcaacatgcaagcaatgagatccgagttcgaacgtcaattagcctctcgcgaagaagatgaggatgatcgtaagaagggactcacttctaaaatccgaaatcttaccgaagagcttgaatctgaacaacgtgctcgtcaagctgcaattgccaacaagaagaagatcgaatctcaaatcagcgagttgactgaaaagaacgaagcttctttgagacaaatcgaagatctttcgcgtcagcttcgcaaagcccaactgggatggaaagatctccagctggatgttacagaagctcgtgctgccatggaagatgctcttgctggacaaagagacgcagagaagagagctcgggcttcggaggatgagatcaagagactcaccgccgatattcaagctgtcagctcttcaaaacgtaaagcagaagctgagcgcgatgaactcattgaagaagtttctagtcttcgcgcttcctcattcagtaatgaggagaagagacgtcttgaagcaaaggtaattgatttggaagaccaactcgacgaggaggcttcggcaaatgaattggcacaagagaaggttcgcaagtcacaacagcaactagaacaaatgacagctgatcttgcaatggaaagaagtgtttgtgagagaacagaatcagataaaattgcattggaacgtgcgaatcgtgatttgaagcaacaacttcaagatgctgaaaataccgctgtggctcgtcttcgtacacaaatcaacgttgcggaagccaaagtttccagtcttgagcaacagctttcattggaagagcaggataagatgcgtcaaggcagaaccctgcgacgaatggaaacgaaaatggcagagatgcagcaaatgctcgaggaagagaagagacaaggtgaaagtaatcgtcaggctgttgatcgtcagaatgctagaatccgtcaacttcgtacacagttggaagatactgaggcagagcgagaccgtttgacaaacaagttgaaggacgagcggagaagagctgaagagatgactgatctcaatgagacactctccagagatgtttctttattgaaacagcgtgaaacgacagctcgtagaacacctggattgattggacatcgtgaatcgagaagatttggaagcaacaccagccttgcacgagatgaattccgtggatcagctctcacaaacgaaatgtcaccatctgaccgtccagcttctcgtctcacatctggaaccggatcccaacttggcaacaccgatgaagaccgcgactcagttcgcaactaa
  Experiment: Laboratory: JH
    Date: 01 Apr 2003 00:00:00
    Genotype: itIs153[pie-1::GFP::PAR-2]
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F20G4.3 Inferred_automatically: RNAi_primary
    Gene: WBGene00003777 Inferred_automatically: RNAi_primary
    Transcript: F20G4.3.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00005735
  Phenotype: WBPhenotype:0000961 Remark: Class I. Embryos in this class do not undergo ruffling, pseudocleavage or cytokinesis. Pronuclei form and migrate normally in these embryos, eventually resulting in the formation of a symmetric spindle. GFP:PAR-2 did not become visibly enriched on the cortex (n=3), and instead was maintained throughout the cytoplasm and also on foci around centrosomes. Class II and Class III. These embryos developed cytokinesis furrows that were either transient (Class II) or lead to the formation of two equal size cells (Class III). Unlike Class I embryos, however, Class II and Class III embryos developed one or two patches of GFP:PAR-2 at the cortex by pronuclear meeting (n=6). In all nmy-2(RNAi) embryos (n=8), GFP:PAR-2 was also detected in foci around the pronuclei or spindle. In four out of eight time-lapse analyses, GFP:PAR-2 appeared to shuttle from the cortex to the nearest pronucleus or centrosome.
  Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.
  Method: RNAi