WormBase Tree Display for RNAi: WBRNAi00088019
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WBRNAi00088019 | Homol | Homol_homol | C12D8:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_C12D8.10 | |||
Experiment | Laboratory | HT | |||
Date | 14 May 2010 00:00:00 | ||||
Genotype | daf-16(mgDf50); daf-16a::gfp | ||||
Treatment | The daf-16(mgDf50) mutants carrying high copies of daf-16a::gfp transgene were maintained at 15 degrees Celsius on RNAi plates for two generations. The plates were then transferred to 20 degrees Celsius for 18 hours. About 15-20 L4 worms were then mounted on slides and visualized by fluorescence microscopy. | ||||
Temperature | 20 | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | C12D8.10a | Inferred_automatically | RNAi_primary | |
C12D8.10c | Inferred_automatically | RNAi_primary | |||
C12D8.10b | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00000102 | Inferred_automatically | RNAi_primary | ||
Transcript | C12D8.10c.1 | Inferred_automatically | RNAi_primary | ||
C12D8.10b.1 | Inferred_automatically | RNAi_primary | |||
C12D8.10a.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Interaction | WBInteraction000518021 | ||||
Reference | WBPaper00036474 | ||||
Phenotype | WBPhenotype:0000679 | Remark | akt-1(RNAi) resulted in a significant increase in nuclear localization of the DAF-16A::GFP product from the daf-16a::gfp transgene. | ||
Remark | akt-1(RNAi); Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
Method | RNAi |