WormBase Tree Display for RNAi: WBRNAi00088025
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| WBRNAi00088025 | Homol | Homol_homol | CHROMOSOME_X:RNAi | ||
|---|---|---|---|---|---|
| C12D8:RNAi | |||||
| Sequence_info | PCR_product | mv_F28H6.1 | |||
| sjj_C12D8.10 | |||||
| Experiment | Laboratory | HT | |||
| Date | 14 May 2010 00:00:00 | ||||
| Genotype | daf-16(mgDf50); daf-16d/f::gfp | ||||
| Treatment | The daf-16(mgDf50) mutants carrying low copies of daf-16d/f::gfp transgene were maintained at 15 degrees Celsius on RNAi plates for two generations. The plates were then transferred to 20 degrees Celsius for 18 hours. About 15-20 L4 worms were then mounted on slides and visualized by fluorescence microscopy. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | C12D8.10a | Inferred_automatically | RNAi_primary | |
| C12D8.10c | Inferred_automatically | RNAi_primary | |||
| F28H6.1b | Inferred_automatically | RNAi_primary | |||
| F28H6.1a | Inferred_automatically | RNAi_primary | |||
| C12D8.10b | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00000103 | Inferred_automatically | RNAi_primary | ||
| WBGene00000102 | Inferred_automatically | RNAi_primary | |||
| Transcript | F28H6.1a.1 | Inferred_automatically | RNAi_primary | ||
| C12D8.10c.1 | Inferred_automatically | RNAi_primary | |||
| F28H6.1b.1 | Inferred_automatically | RNAi_primary | |||
| C12D8.10b.1 | Inferred_automatically | RNAi_primary | |||
| C12D8.10a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000518020 | ||||
| Reference | WBPaper00036474 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | akt-1(RNAi);akt-2(RNAi) resulted in a significant increase in nuclear localization of the DAF-16D/F::GFP product from the daf-16d/f::gfp transgene, compared to akt-1(RNAi) alone, suggesting that akt-2(RNAi) enhances the increased DAF-16D/F::GFP nuclear localization of akt-1(RNAi). | ||
| Remark | akt-1/2(RNAi); Exact sequence used for akt-1 RNAi not stated by authors, Ahringer laboratory clone used for curation. Exact sequence used for akt-2 RNAi not stated by authors, Vidal laboratory clone used for curation. | ||||
| Method | RNAi |
