WormBase Tree Display for RNAi: WBRNAi00088798
expand all nodes | collapse all nodes | view schema
| WBRNAi00088798 | Homol | Homol_homol | F38H4:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F38H4.9 | |||
| Experiment | Laboratory | OC | |||
| Date | 28 Feb 2011 00:00:00 | ||||
| Genotype | unc-119(ed3)III; fem-1(hc17)IV; ItIs33[pOD224; pie-1-gfpTEV-stag::sas-6 genomic; unc-119(+)genomic] | ||||
| Treatment | To feed dsRNA, L4 larvae were placed on a lawn of bacteria that carried a plasmid for inducible expression of let-92 (Source Bioscience plc, Nottingham, UK) and allowed them to feed for 1 day at 25 degrees Celsius. The SAS-6 recruitment assay was performed essentially as described. Briefly, wild-type males were mated overnight to OD103 females on RNAi plates at 25 degrees Celsius. The next day embryos were immunostained using the a-SAS-4 antibody to mark centrioles and the anti-GFP antibody to detect GFP-SAS-6. | ||||
| Temperature | 25 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F38H4.9 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00002363 | Inferred_automatically | RNAi_primary | ||
| Transcript | F38H4.9.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00038325 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | let-92(RNAi) prevented transgenic GFP::SAS-6 from properly localizing to centrioles | ||
| WBPhenotype:0001403 | Remark | let-92(RNAi) prevented transgenic GFP::SAS-6 from properly localizing to centrioles | |||
| Remark | let-92 RNAi | ||||
| Method | RNAi |
