WormBase Tree Display for RNAi: WBRNAi00088833

WBRNAi00088833 Homol: Homol_homol: F53G12:RNAi
  Sequence_info: DNA_text: atgggctctcgtgacgatgaatacgactacttgttcaaggttgttctgattggagactcaggcgtcggaaagtcgaatctcctgtctcgtttcacaagaaatgagttcaacttggaatcaaaatcaacaatcggagtcgagtttgccacgagaagcatctcggtagaaggcaagacagtgaaggctcaaatttgggatactgctggacaggaacgttaccgtgccatcacatccgcttactatcgtggggctgtcggagctctcctagtctacgacatcgctaagcatgtgacgtacgagaatgttgagcgatggttgaaggagcttcgtgatcacgccgatcagaacattgtgattatgttggtcggaaacaagagcgacttgcgccatttgcgtgcagttccaacagacgaggccaagatctacgccgaaagaaatcaattgtcgtttattgaaacatctgccctcgacagcaccaacgttgaagcagctttcactaatatcctgacggaaatctacaaatcagtatccaacaagcatgtaggaactgacagacaaggatatggcggtggcagtggtacaatcattccttcgccagcgtccgacccaccaaagaagcagtgttgcatcccataa
  Experiment: Laboratory: MAD
    Date: 14 Mar 2011 00:00:00
    Genotype: [GFP::TBB-2; GFP::PAR-2; mCherry::PAR-6]
    Treatment: L4-stage hermaphrodites were fed bacteria expressing double-stranded RNA. Complete depletion of RAB-11 results in decreased brood size and sterility due to defects in embryogenesis and germline membrane organization. Therefore, a 1:1 ratio of bacterial cultures containing rab-11(RNAi) plasmid and L4440 vector were mixed together to reduce the RNAi effect. RNAi experiments were performed for 20-25 hours at 20 degrees C
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F53G12.1 Inferred_automatically: RNAi_primary
    Gene: WBGene00004274 Inferred_automatically: RNAi_primary
    Transcript: F53G12.1.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00038375
  Phenotype: WBPhenotype:0000034 Remark: rab-11(RNAi) embryos displayed rotated boundaries between PAR-6- and PAR-2- occupied domains during the polarity maintenance phase of the first cell cycle (Fig. 4H and 5D). RAB-11 knockdown also affected establishment phase. Polarity failed to completely establish in rab-11(RNAi) embryos (Fig. 4G).
    WBPhenotype:0000679 Remark: rab-11(RNAi) embryos displayed rotated boundaries between PAR-6- and PAR-2- occupied domains during the polarity maintenance phase of the first cell cycle (Fig. 4H and 5D). RAB-11 knockdown also affected establishment phase. Polarity failed to completely establish in rab-11(RNAi) embryos (Fig. 4G).
    WBPhenotype:0001588 Remark: rab-11(RNAi) led to shorter astral microtubules at the nuclear envelope breakdown of the first cell cycle.
  Remark: rab-11(RNAi)
  Method: RNAi