WormBase Tree Display for RNAi: WBRNAi00088835

WBRNAi00088835 Homol: Homol_homol: F22B5:RNAi
      Y73F8A:RNAi
      ZC581:RNAi
      Y55B1BM:RNAi
      Y57G11A:RNAi
      Y57E12B:RNAi
      Y53F4A:RNAi
      Y97E10B:RNAi
      ZK1086:RNAi
      Y40H7A:RNAi
  Sequence_info: PCR_product: sjj_F22B5.7
  Experiment: Laboratory: MAD
    Date: 14 Mar 2011 00:00:00
    Genotype: [GFP::TBB-2; GFP::PAR-2; mCherry::PAR-6]
    Treatment: L4-stage hermaphrodites were fed bacteria expressing double-stranded RNA. RNAi experiments were performed for 20-25 hours at 20 degrees C
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F22B5.7 Inferred_automatically: RNAi_primary
    Gene: WBGene00006994 Inferred_automatically: RNAi_primary
    Transcript: F22B5.7.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00038375
  Phenotype: WBPhenotype:0000034 Remark: In zyg-9(RNAi) embryos, polarity establishment was not affected, but during maintenance phase the boundaries between PAR-6 and PAR-2 were not perpendicular to the AP axis (Fig. 4M-O and 5F; also see Video S11).
    WBPhenotype:0000679 Remark: In zyg-9(RNAi) embryos, polarity establishment was not affected, but during maintenance phase the boundaries between PAR-6 and PAR-2 were not perpendicular to the AP axis (Fig. 4M-O and 5F; also see Video S11).
    WBPhenotype:0000761 Remark: In zyg-9(RNAi) embryos, pronuclear migration and rotation were altered, which resulted in centrosomes remaining in the posterior of the embryos and failing to rotate. In addition, the migration of oocyte pronuclei was delayed. Paternal pronuclei underwent nuclear envelope breakdown prior to pronuclear meeting and formed the spindle without maternal chromosomes. The mitotic spindle set up near the posterior, perpendicular to the AP axis. The result of this posteriorly placed spindle was the formation of two furrows, one in the anterior at the borders between the PAR-6 and PAR-2 domains, and a second furrow invaginating in the posterior between the two centrosomes (Fig. 4L,O).
    WBPhenotype:0000766 Remark: In zyg-9(RNAi) embryos, pronuclear migration and rotation were altered, which resulted in centrosomes remaining in the posterior of the embryos and failing to rotate. In addition, the migration of oocyte pronuclei was delayed. Paternal pronuclei underwent nuclear envelope breakdown prior to pronuclear meeting and formed the spindle without maternal chromosomes. The mitotic spindle set up near the posterior, perpendicular to the AP axis. The result of this posteriorly placed spindle was the formation of two furrows, one in the anterior at the borders between the PAR-6 and PAR-2 domains, and a second furrow invaginating in the posterior between the two centrosomes (Fig. 4L,O).
    WBPhenotype:0001152 Remark: In zyg-9(RNAi) embryos, pronuclear migration and rotation were altered, which resulted in centrosomes remaining in the posterior of the embryos and failing to rotate. In addition, the migration of oocyte pronuclei was delayed. Paternal pronuclei underwent nuclear envelope breakdown prior to pronuclear meeting and formed the spindle without maternal chromosomes. The mitotic spindle set up near the posterior, perpendicular to the AP axis. The result of this posteriorly placed spindle was the formation of two furrows, one in the anterior at the borders between the PAR-6 and PAR-2 domains, and a second furrow invaginating in the posterior between the two centrosomes (Fig. 4L,O).
    WBPhenotype:0001158 Remark: In zyg-9(RNAi) embryos, pronuclear migration and rotation were altered, which resulted in centrosomes remaining in the posterior of the embryos and failing to rotate. In addition, the migration of oocyte pronuclei was delayed. Paternal pronuclei underwent nuclear envelope breakdown prior to pronuclear meeting and formed the spindle without maternal chromosomes. The mitotic spindle set up near the posterior, perpendicular to the AP axis. The result of this posteriorly placed spindle was the formation of two furrows, one in the anterior at the borders between the PAR-6 and PAR-2 domains, and a second furrow invaginating in the posterior between the two centrosomes (Fig. 4L,O).
    WBPhenotype:0001588 Remark: In zyg-9(RNAi) embryos, the lengths of astral microtubules at nuclear envelope breakdown (of the first cell cycle) were significantly shorter than in control animals.
    WBPhenotype:0002501 Remark: In zyg-9(RNAi) embryos, pronuclear migration and rotation were altered, which resulted in centrosomes remaining in the posterior of the embryos and failing to rotate. In addition, the migration of oocyte pronuclei was delayed. Paternal pronuclei underwent nuclear envelope breakdown prior to pronuclear meeting and formed the spindle without maternal chromosomes. The mitotic spindle set up near the posterior, perpendicular to the AP axis. The result of this posteriorly placed spindle was the formation of two furrows, one in the anterior at the borders between the PAR-6 and PAR-2 domains, and a second furrow invaginating in the posterior between the two centrosomes (Fig. 4L,O).
  Remark: zyg-9(RNAi)
  Method: RNAi