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WormBase Tree Display for RNAi: WBRNAi00092269

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Name Class

WBRNAi00092269HomolHomol_homolE04F6:RNAi
Sequence_infoPCR_productsjj_E04F6.5
ExperimentLaboratoryJZF
Date26 Jun 2012 00:00:00
StrainWBStrain00000001
TreatmentRNAi treatments were carried out by adding synchronized eggs to plates containing bacteria with an empty vector (L4440) and then transferring the larva to plates seeded with the RNAi bacteria 3 days later (L4/Young Adult). Nematodes were moved to plates with fresh RNAi bacteria every 2 days until reproduction was completed and then moved to new plates every 5-7 days for the rest of the life span analysis. Viability of the nematodes was scored every 2-3 days. Similar results were obtained when treating worms with RNAi from hatching.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneE04F6.5aInferred_automaticallyRNAi_primary
GeneWBGene00017125Inferred_automaticallyRNAi_primary
TranscriptE04F6.5a.1Inferred_automaticallyRNAi_primary
E04F6.5a.2Inferred_automaticallyRNAi_primary
E04F6.5bInferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000520154
ReferenceWBPaper00041315
Phenotype_not_observedWBPhenotype:0000039Remarkacdh-12 RNAi did not significantly affect wild type (N2) life span
Remark(Figure 6, Table S2a) acdh-12 RNAi
MethodRNAi