WormBase Tree Display for RNAi: WBRNAi00092414
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| WBRNAi00092414 | Homol | Homol_homol | Y71A12B:RNAi | ||
|---|---|---|---|---|---|
| Y63D3A:RNAi | |||||
| Sequence_info | DNA_text | ATGGTGCATTCAAACGGAGCCATCACGAGCACAATTCTGAAGGCTCGTCACGCCGACGTCGTCCGCAAGACGTCACTTCACCACGCCAACGACTTGACACTCATCGATCTCGTGCTCAATGTGCAGCGGCTTTTGGCTCTCCCATCCGACGCGAATTTCGTGTTGAAGTACAAGGATGAAGgtaggaattgcactgaaaattgcgattttaattcaatttttgtcaattttagAAGGCGATTTGGTGACACTCGCCGAGGATAGCGACCTCCTGCTGGCTCTTCACACGTCTGGAGCCACGCTCGACGTGACCGTCGTTGTCGACTCGAGAGCTCGTGAGGCTGTGCACGATGTGCAGAAGCAAGTCGAGCAGATTAAG | |||
| Experiment | Laboratory | MSN | |||
| Date | 07 Feb 2011 00:00:00 | ||||
| Treatment | Early-L4-stage hermaphrodites were soaked in dsRNA for 24 hours at 20 degrees Celsius. Animals were allowed to recover for 48 hours before analysis. | ||||
| Temperature | 20 | ||||
| Delivered_by | Soaking | ||||
| Inhibits | Predicted_gene | Y63D3A.5 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00006565 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y63D3A.5.2 | Inferred_automatically | RNAi_primary | ||
| Y63D3A.5.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000520198 | ||||
| Reference | WBPaper00038310 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | tfg-1 RNAi resulted in disrupted plasma membrane localization of the GFP-SNB-1 transgene product in distal gonads. Instead of trafficking to the plasma membrane, GFP-SNB-1 was often found mislocalized to the endoplasmic reticulum suggesting it was trapped their during the protein secretion process. | ||
| Remark | (Figure 5c) tfg-1 RNAi. Authors report testing three different dsRNA sequences to knockdown tfg-1 and all produced similar results. One selected for curation. | ||||
| Method | RNAi |
