WormBase Tree Display for RNAi: WBRNAi00092418
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| WBRNAi00092418 | Homol | Homol_homol | ZK512:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | CTTCTGGTTTCGATATGAGTGACTGGAATAATCCTTACAATGCTTCACCTCCGTCTTCTAGAGGAGGCGATGATGACGCTTCATCCGTTAACCATTCAAGgtcagtgaatacaagaatttcacacgccgatcgatatcttttcatcgcttttttttcagGCCTCGACGCAGTCGGCTGGACAACGATATTCCACAACCAAGAAGACCCATTCTTATTCAACCAGCTCGCCCAGTATCTCAAAAGAGTAACCGGCAGGGTACTGGAATGAGTAATGGTTCAAGAGGCCTCAACTCAACTTTCAATGGATATGACCGTACTTATTCAAGgtttaaaacaagggagtttcttcttctaattatggaaatttgcagATATCACCAAAACTCTTCACGTGGCCCAAGCGAAGGTTTCTCTGGTGCTCCTTCAGCTCGAAATGCATCTGGATATGCGAGTGATTACGCGAATAGTCGTGCTGGTGTGGGATTGCTGCCGAACAACCACCGTGAGCCTGTGAGACCACGATCAACTGCTGCTGAAAGATATGCGAACGCATCTTCAATGAGAAATGGTTTTGTATATGATAGTGGAGAGTCTGATAAGACTTCAGAAGAGCTGGAAGAAGACGAAGAAGAGGAAGAAGTACGAAATTTTTATATGGAAGGACGAGCACAAGGCTCCAGGAGTGTAACAAATACCCTTGCCTCTGAAGTTTATAATAGCGAAAGTGAAAGCTATTACTACGGAGTTGTGAAACTTGGCTCTGCTATTGTTGATCATGTGTTCCGAACGATGCCTCCTCCGGAAAAATACTATAAGATGCCGCCAATCGACCGTGTCGCTTATGTATTCTACTGTGCCGTTAATAACAAACCTTACAACAATATTGATGAGTTTCACGTAATTTTTAATCGTGAATTCTACTCCTACCGCGGGTATGGTGATTCTAAAGATCTGGCACTTTTTAAGgtaagattactgacaaaattgaattgcttcatattttaaattacagGTTTGC | |||
| Experiment | Laboratory | MSN | |||
| Date | 07 Feb 2011 00:00:00 | ||||
| Genotype | mCherry::RAB-5; GFP::RAB-6 | ||||
| Treatment | Early-L4-stage hermaphrodites were soaked in dsRNA for 24 hours at 20 degrees Celsius. Animals were allowed to recover for 48 hours before analysis. | ||||
| Temperature | 20 | ||||
| Delivered_by | Soaking | ||||
| Inhibits | Predicted_gene | ZK512.5b | Inferred_automatically | RNAi_primary | |
| ZK512.5a | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00013985 | Inferred_automatically | RNAi_primary | ||
| Transcript | ZK512.5a.1 | Inferred_automatically | RNAi_primary | ||
| ZK512.5b.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000520202 | ||||
| Reference | WBPaper00038310 | ||||
| Phenotype | WBPhenotype:0000679 | Remark | Following depletion of SEC-16 by RNAi, RAB-6 accumulated on fewer, large punctate structures in the germline that also harbored RAB-5, indicating that RAB-6 localization was shifted from the Golgi to enlarged early endosomes. | ||
| Remark | (Table S1) sec-16 RNAi. Authors report testing three different dsRNA sequences to knockdown sec-16 and all produced similar results. One selected for curation. | ||||
| Method | RNAi |
