WormBase Tree Display for RNAi: WBRNAi00092543
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| WBRNAi00092543 | Homol | Homol_homol | R13H8:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_R13H8.1 | |||
| Experiment | Laboratory | KM | |||
| Date | 16 Mar 2010 00:00:00 | ||||
| Genotype | ogt-1(ok430); oga-1(ok1207) | ||||
| Treatment | For lifespan assays, strains were maintained at 15 degrees Celsius. Synchronous age populations were obtained by placing 3-5 gravid adult hermaphrodites per RNAi plate overnight to lay eggs. Adults were removed the following morning, and progeny were allowed to develop at 15 degrees Celsius. After the final larval molt, at day 0 of adulthood, animals were transferred to RNAi plates containing 400mM FUDR (Sigma) and placed at 20 degrees Celsius for the duration of the lifespan assay. Death was scored by the inability to respond to a gentle mechanical stimulus from a platinum wire. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene (11) | ||||
| Gene | WBGene00000912 | Inferred_automatically | RNAi_primary | ||
| Transcript (11) | |||||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00036090 | ||||
| Phenotype | WBPhenotype:0001171 | ||||
| Remark | (Dataset_S5) daf-16 RNAi | ||||
| Method | RNAi |
