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WormBase Tree Display for RNAi: WBRNAi00092706

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Name Class

WBRNAi00092706HomolHomol_homolC10H11:RNAi
C06A5:RNAi
Sequence_infoPCR_productsjj_C06A5.7
sjj_C10H11.9
ExperimentLaboratorySU
Date06 Jun 2012 00:00:00
Genotypehmp-1(fe4); jcIs24 [jac-1p::GFP]
TreatmentdsRNA was injected at 2 micrograms per microliter, and let-502 at 1 microgram per microliter, into one gonad arm per worm. To score for embryonic and early larval lethality, worms were cultured for 12-16 hours after injection and then singled onto new plates. The embryos produced over the next 24 hours were scored for lethality. To obtain embryos for phenotypic analysis, worms were cut transversely through the vulva at 24-30 hours post-injection and extruded embryos were collected.
Delivered_byInjection
InhibitsPredicted_geneC06A5.7aInferred_automaticallyRNAi_primary
C10H11.9Inferred_automaticallyRNAi_primary
C06A5.7bInferred_automaticallyRNAi_primary
GeneWBGene00006823Inferred_automaticallyRNAi_primary
WBGene00002694Inferred_automaticallyRNAi_primary
TranscriptC06A5.7b.1Inferred_automaticallyRNAi_primary
C06A5.7a.2Inferred_automaticallyRNAi_primary
C10H11.9.1Inferred_automaticallyRNAi_primary
C06A5.7a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000520288
ReferenceWBPaper00041277
PhenotypeWBPhenotype:0000679Remarklet-502 RNAi partially suppresses the adherens junction defects and JAC-1::GFP mislocalization of hmp-1(fe4);unc-94(RNAi) animals
WBPhenotype:0001745Remarklet-502 RNAi partially suppresses the adherens junction defects and JAC-1::GFP mislocalization of hmp-1(fe4);unc-94(RNAi) animals
Remark(Figure 3D, S3E) unc-94, let-502 double RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi