WormBase Tree Display for RNAi: WBRNAi00095860
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WBRNAi00095860 | Homol | Homol_homol | F32H2:RNAi | |
---|---|---|---|---|
Sequence_info | PCR_product | sjj_F32H2.5 | ||
Experiment | Laboratory | GR | ||
Date | 23 Feb 2012 00:00:00 | |||
Strain | WBStrain00000264 | |||
Treatment | For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates. | |||
Delivered_by | Bacterial_feeding | |||
Inhibits (3) | ||||
Species | Caenorhabditis elegans | |||
Reference | WBPaper00040984 | |||
Phenotype_not_observed | WBPhenotype:0001236 | Remark | RNAi does not result in activation of the irg-1::GFP irg-1::GFP reporter gene | |
Remark | (Table S2A) fasn-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation. | |||
Method | RNAi |