WormBase Tree Display for RNAi: WBRNAi00096557
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WBRNAi00096557 | Homol | Homol_homol | W06B3:RNAi | |
---|---|---|---|---|
Y69A2AR:RNAi | ||||
Y17D7C:RNAi | ||||
W08F4:RNAi | ||||
Sequence_info | PCR_product | sjj_W06B3.1 | ||
Experiment | Laboratory | GR | ||
Date | 23 Feb 2012 00:00:00 | |||
Strain | WBStrain00034068 | |||
Treatment | For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates. | |||
Delivered_by | Bacterial_feeding | |||
Inhibits (3) | ||||
Species | Caenorhabditis elegans | |||
Reference | WBPaper00040984 | |||
Phenotype_not_observed | WBPhenotype:0001236 | Remark | RNAi does not result in activation of the hsp-6::GFP reporter gene | |
Remark | (Table S2B) W06B3.1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation. | |||
Method | RNAi |