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WormBase Tree Display for RNAi: WBRNAi00097862

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Name Class

WBRNAi00097862HomolHomol_homolW06B4:RNAi
Sequence_infoPCR_productsjj_W06B4.3
ExperimentLaboratoryJCP
Date29 Mar 2010 00:00:00
GenotypePced-1::CCZ-1::YFP
TreatmentSynchronized L1 larvae were spotted onto feeding plates and incubated at 15 degrees Celsius until the gonads of adults and embryos of the next generation could be scored.
Temperature15
Delivered_byBacterial_feeding
InhibitsPredicted_geneW06B4.3Inferred_automaticallyRNAi_primary
GeneWBGene00021058Inferred_automaticallyRNAi_primary
TranscriptW06B4.3.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036342
PhenotypeWBPhenotype:0000679Remarkvps-18 RNAi resulted in visible fluorescent halos of CCZ-1::YFP in phagosomes engulfing apoptotic cell corpses (as in wild type), albeit to a lesser extent than wild type. The presence of the CCZ-1::YFP halos suggests that CCZ-1 acts upstream of VPS-18 in the phagosome maturation pathway.
WBPhenotype:0001846Remarkvps-18 RNAi resulted in visible fluorescent halos of CCZ-1::YFP in phagosomes engulfing apoptotic cell corpses (as in wild type), albeit to a lesser extent than wild type. The presence of the CCZ-1::YFP halos suggests that CCZ-1 acts upstream of VPS-18 in the phagosome maturation pathway.
Remark(Figure 3B, 3C) vps-18 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi