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WormBase Tree Display for RNAi: WBRNAi00099161

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Name Class

WBRNAi00099161HomolHomol_homolB0564:RNAi
Sequence_infoPCR_productsjj_B0564.1
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneB0564.1aInferred_automaticallyRNAi_primary
B0564.1cInferred_automaticallyRNAi_primary
B0564.1bInferred_automaticallyRNAi_primary
GeneWBGene00007201Inferred_automaticallyRNAi_primary
WBGene00044083Inferred_automaticallyRNAi_primary
TranscriptB0564.1b.1Inferred_automaticallyRNAi_primary
B0564.1a.1Inferred_automaticallyRNAi_primary
B0564.1a.3Inferred_automaticallyRNAi_primary
B0564.1b.2Inferred_automaticallyRNAi_primary
B0564.1a.2Inferred_automaticallyRNAi_primary
B0564.1c.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the fkh-6::GFP transgene in hermaphrodite animals
PenetranceRange84
Remark(Table S1) tin-9.2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi