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WormBase Tree Display for RNAi: WBRNAi00099191

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Name Class

WBRNAi00099191HomolHomol_homolF34D10:RNAi
Sequence_infoPCR_productsjj_F34D10.3
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneF34D10.3Inferred_automaticallyRNAi_primary
F34D10.2Inferred_automaticallyRNAi_primary
GeneWBGene00009373Inferred_automaticallyRNAi_primary
WBGene00009372Inferred_automaticallyRNAi_primary
TranscriptF34D10.3.1Inferred_automaticallyRNAi_primary
F34D10.2.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the fkh-6::GFP transgene in hermaphrodite animals
PenetranceRange94
Remark(Table S1) F34D10.2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi