WormBase Tree Display for RNAi: WBRNAi00099220
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WBRNAi00099220 | Homol | Homol_homol | Y22D7AL:RNAi | ||
---|---|---|---|---|---|
Sequence_info | PCR_product | mv_AAA28077 | |||
Experiment | Laboratory | DZ | |||
Date | 11 Mar 2010 00:00:00 | ||||
Genotype | rrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP] | ||||
Treatment | Eggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced. | ||||
Delivered_by | Bacterial_feeding | ||||
Inhibits | Predicted_gene | Y22D7AL.5b | Inferred_automatically | RNAi_primary | |
Y22D7AL.5a | Inferred_automatically | RNAi_primary | |||
Y22D7AL.5c | Inferred_automatically | RNAi_primary | |||
Gene | WBGene00002025 | Inferred_automatically | RNAi_primary | ||
Transcript | Y22D7AL.5c.1 | Inferred_automatically | RNAi_primary | ||
Y22D7AL.5a.1 | Inferred_automatically | RNAi_primary | |||
Y22D7AL.5a.2 | Inferred_automatically | RNAi_primary | |||
Y22D7AL.5b.1 | Inferred_automatically | RNAi_primary | |||
Species | Caenorhabditis elegans | ||||
Reference | WBPaper00036019 | ||||
Phenotype | WBPhenotype:0000961 | Remark | RNAi resulted in abnormal expression of the fkh-6::GFP transgene in hermaphrodite animals | ||
Penetrance | Range | 96 | |||
Remark | (Table S1) hsp-60 RNAi. Exact sequence used for RNAi not stated by authors, Vidal laboratory clone used for curation. | ||||
Method | RNAi |