Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for RNAi: WBRNAi00099220

expand all nodes | collapse all nodes | view schema

Name Class

WBRNAi00099220HomolHomol_homolY22D7AL:RNAi
Sequence_infoPCR_productmv_AAA28077
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneY22D7AL.5bInferred_automaticallyRNAi_primary
Y22D7AL.5aInferred_automaticallyRNAi_primary
Y22D7AL.5cInferred_automaticallyRNAi_primary
GeneWBGene00002025Inferred_automaticallyRNAi_primary
TranscriptY22D7AL.5c.1Inferred_automaticallyRNAi_primary
Y22D7AL.5a.1Inferred_automaticallyRNAi_primary
Y22D7AL.5a.2Inferred_automaticallyRNAi_primary
Y22D7AL.5b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the fkh-6::GFP transgene in hermaphrodite animals
PenetranceRange96
Remark(Table S1) hsp-60 RNAi. Exact sequence used for RNAi not stated by authors, Vidal laboratory clone used for curation.
MethodRNAi