WormBase Tree Display for RNAi: WBRNAi00099239
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WBRNAi00099239 | Homol | Homol_homol | C25A11:RNAi | |||
---|---|---|---|---|---|---|
Sequence_info | PCR_product | sjj_C25A11.4 | ||||
Experiment | Laboratory | DZ | ||||
Date | 11 Mar 2010 00:00:00 | |||||
Genotype | rrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP] | |||||
Treatment | Eggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced. | |||||
Delivered_by | Bacterial_feeding | |||||
Inhibits | Predicted_gene (13) | |||||
Gene | WBGene00000100 | Inferred_automatically | RNAi_primary | |||
Transcript (16) | ||||||
Species | Caenorhabditis elegans | |||||
Reference | WBPaper00036019 | |||||
Phenotype | WBPhenotype:0000961 | Remark | RNAi resulted in abnormal expression of the fkh-6::GFP transgene in hermaphrodite animals | |||
Penetrance | Range | 50 | 100 | |||
Remark | (Table S1) ajm-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | |||||
Method | RNAi |