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WormBase Tree Display for RNAi: WBRNAi00099536

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Name Class

WBRNAi00099536HomolHomol_homolT05G5:RNAi
Sequence_infoPCR_productsjj_T05G5.3
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs3 [fkh-6::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneT05G5.3Inferred_automaticallyRNAi_primary
GeneWBGene00000405Inferred_automaticallyRNAi_primary
TranscriptT05G5.3.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the fkh-6::GFP transgene in male animals
PenetranceRange88
Remark(Table S1, Figure 2M) cdk-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi