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WormBase Tree Display for RNAi: WBRNAi00099570

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Name Class

WBRNAi00099570HomolHomol_homolC12C8:RNAi
Sequence_infoPCR_productsjj_C12C8.3
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs1 [K09C8.2::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC12C8.3bInferred_automaticallyRNAi_primary
C12C8.3aInferred_automaticallyRNAi_primary
GeneWBGene00003026Inferred_automaticallyRNAi_primary
TranscriptC12C8.3b.1Inferred_automaticallyRNAi_primary
C12C8.3a.1Inferred_automaticallyRNAi_primary
C12C8.3b.2Inferred_automaticallyRNAi_primary
C12C8.3a.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the K09C8.2::GFP transgene in male animals
PenetranceRange14
Remark(Table S1) lin-41 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi