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WormBase Tree Display for RNAi: WBRNAi00099577

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Name Class

WBRNAi00099577HomolHomol_homolC37C3:RNAi
Sequence_infoPCR_productsjj_C37C3.6
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs1 [K09C8.2::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC37C3.6bInferred_automaticallyRNAi_primary
C37C3.6cInferred_automaticallyRNAi_primary
C37C3.6aInferred_automaticallyRNAi_primary
GeneWBGene00003242Inferred_automaticallyRNAi_primary
TranscriptC37C3.6b.1Inferred_automaticallyRNAi_primary
C37C3.6a.2Inferred_automaticallyRNAi_primary
C37C3.6a.1Inferred_automaticallyRNAi_primary
C37C3.6c.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the K09C8.2::GFP transgene in male animals
PenetranceRange63
Remark(Table S1) ppn-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi