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WormBase Tree Display for RNAi: WBRNAi00099589

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Name Class

WBRNAi00099589HomolHomol_homolF19B6:RNAi
Sequence_infoPCR_productsjj_F19B6.1
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs1 [K09C8.2::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneF19B6.1bInferred_automaticallyRNAi_primary
F19B6.1dInferred_automaticallyRNAi_primary
F19B6.1cInferred_automaticallyRNAi_primary
F19B6.1aInferred_automaticallyRNAi_primary
GeneWBGene00008948Inferred_automaticallyRNAi_primary
TranscriptF19B6.1c.1Inferred_automaticallyRNAi_primary
F19B6.1a.2Inferred_automaticallyRNAi_primary
F19B6.1d.1Inferred_automaticallyRNAi_primary
F19B6.1b.1Inferred_automaticallyRNAi_primary
F19B6.1a.1Inferred_automaticallyRNAi_primary
F19B6.1a.3Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the K09C8.2::GFP transgene in male animals
PenetranceRange8
Remark(Table S1) F19B6.1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi