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WormBase Tree Display for RNAi: WBRNAi00099721

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Name Class

WBRNAi00099721HomolHomol_homolF19F10:RNAi
Sequence_infoPCR_productsjj_F19F10.9
ExperimentLaboratoryDZ
Date11 Mar 2010 00:00:00
Genotyperrf-3(pk1426); him-8(e1489); ezIs1 [K09C8.2::GFP]
TreatmentEggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced.
Delivered_byBacterial_feeding
InhibitsPredicted_geneF19F10.9Inferred_automaticallyRNAi_primary
GeneWBGene00017605Inferred_automaticallyRNAi_primary
TranscriptF19F10.9.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00036019
PhenotypeWBPhenotype:0000961RemarkRNAi resulted in abnormal expression of the K09C8.2::GFP transgene in male animals
PenetranceRange54
Remark(Table S1) F19F10.9 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi