WormBase Tree Display for RNAi: WBRNAi00099730
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| WBRNAi00099730 | Homol | Homol_homol | F32H2:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F32H2.3 | |||
| Experiment | Laboratory | DZ | |||
| Date | 11 Mar 2010 00:00:00 | ||||
| Genotype | rrf-3(pk1426); him-8(e1489); ezIs1 [K09C8.2::GFP] | ||||
| Treatment | Eggs were recovered from gravid hermaphrodites by hypochlorite treatment, dropped onto RNAi plates, and incubated at 22 degrees Celsius for 3 days. Young adult animals were scored under fluorescence microscopy (Nikon SMZ1500) for missing, abnormal, or sex-inappropriate marker expression. All positive RNAi clones confirmed after retesting were sequenced. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F32H2.3 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004953 | Inferred_automatically | RNAi_primary | ||
| Transcript | F32H2.3.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00036019 | ||||
| Phenotype | WBPhenotype:0000961 | Remark | RNAi resulted in abnormal expression of the K09C8.2::GFP transgene in male animals | ||
| Penetrance | Range | 40 | |||
| Remark | (Table S1) spd-2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
| Method | RNAi |
