WormBase Tree Display for RNAi: WBRNAi00101454
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WBRNAi00101454 | Homol | Homol_homol | C03C10:RNAi | |||
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Sequence_info | DNA_text | ATGGCAGGTGTGATGTCAGTTAAGGACTTCATCGTTGCGACAAAATACAAGCTCATACGAAAAATCGGATCAGGATCATTCGGTGACATTTATGTATCGATCAACGTGACGAACGGCGAGGAGGTGGCCATTAAGCTGGAAAGCAATCGGGCGAGGCACCCGCAGTTGCTGTACGAGTCCAAAGTGTATCGTATCCTACAGGGAGGAGTCGGAATTCCACATATCAGATGGTACGGAACCGAGCGAGAGTACAATGTGCTTGTCATGGATCTTCTCGGTCCTTCATTGGAAGATCTCTTCAATTTCTGCTCCAGAAGATTTACCATGAAGACTGTTCTCATGCTGGCTGACCAGATGATCGGACGTATCGAATATGTGCATGTCAAGAACTTTATTCATCGTGACATAAAGCCAGACAACTTCTTGATGGGAATAGGACGTCACTGCAACAAGCTCTTCTTGATCGACTTCGGATTGGCCAAAAAATACCGTGATTCACGCACCAGAACTCATATTCCATACAGAGAGGACAAGAACTTGACCGGTACCGCCAGATACGCATCCATCAACGCCCACTTGGGAATTGAGCAGTCGAGAAGAGATGACATGGAATCCCTTGGATACGTTTTGATGTACTTCAACCGTGGAACTCTTCCATGGCAAGGATTGAAGGCCGCCACGAAAAAGCAAAAGTATGAGAAGATTTCTGAGAAGAAAATGACGACATCAGTCGAGCACTTGTGCAAAGGATTCCCAGCTGAGTTCCCAATGTACTTGAGCTACACAAGAGGACTTCGTTTCGATGAATCTCCAGATTACATGTACTTGCGGCAACTGTTCAGAATTCTCTTCAGAACGCTCAATCATCAGTACGACTACACATTCGACTGGACAATGCTCAAACAAAAGGCTCAACAATCGCAATCCAGTGGCGTCCCCGGAACCAACACTACTACACAGGGAGCTACCGTTCCATCAGCTGGAGTTCCAGCTGGAGTTGCACCAGGAGGAACTACTCCACAGTAA | ||||
Experiment | Laboratory | CT | ||||
Date | 01 Nov 2010 00:00:00 | |||||
Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted. | |||||
Temperature | 20 | |||||
Delivered_by | Bacterial_feeding | |||||
Inhibits | Predicted_gene | C03C10.1 | Inferred_automatically | RNAi_primary | ||
Gene | WBGene00002202 | Inferred_automatically | RNAi_primary | |||
Transcript | C03C10.1.3 | Inferred_automatically | RNAi_primary | |||
C03C10.1.1 | Inferred_automatically | RNAi_primary | ||||
C03C10.1.2 | Inferred_automatically | RNAi_primary | ||||
Species | Caenorhabditis elegans | |||||
Reference | WBPaper00037838 | |||||
Phenotype | WBPhenotype:0000022 | Remark | At the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile. | |||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
WBPhenotype:0000164 | Remark | At the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile. | ||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
WBPhenotype:0000688 | Remark | At the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile. | ||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
WBPhenotype:0000697 | Remark | At the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile. | ||||
EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
Remark | (Data not shown) kin-19 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | |||||
Method | RNAi |