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WormBase Tree Display for RNAi: WBRNAi00101454

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Name Class

WBRNAi00101454HomolHomol_homolC03C10:RNAi
Sequence_infoDNA_textATGGCAGGTGTGATGTCAGTTAAGGACTTCATCGTTGCGACAAAATACAAGCTCATACGAAAAATCGGATCAGGATCATTCGGTGACATTTATGTATCGATCAACGTGACGAACGGCGAGGAGGTGGCCATTAAGCTGGAAAGCAATCGGGCGAGGCACCCGCAGTTGCTGTACGAGTCCAAAGTGTATCGTATCCTACAGGGAGGAGTCGGAATTCCACATATCAGATGGTACGGAACCGAGCGAGAGTACAATGTGCTTGTCATGGATCTTCTCGGTCCTTCATTGGAAGATCTCTTCAATTTCTGCTCCAGAAGATTTACCATGAAGACTGTTCTCATGCTGGCTGACCAGATGATCGGACGTATCGAATATGTGCATGTCAAGAACTTTATTCATCGTGACATAAAGCCAGACAACTTCTTGATGGGAATAGGACGTCACTGCAACAAGCTCTTCTTGATCGACTTCGGATTGGCCAAAAAATACCGTGATTCACGCACCAGAACTCATATTCCATACAGAGAGGACAAGAACTTGACCGGTACCGCCAGATACGCATCCATCAACGCCCACTTGGGAATTGAGCAGTCGAGAAGAGATGACATGGAATCCCTTGGATACGTTTTGATGTACTTCAACCGTGGAACTCTTCCATGGCAAGGATTGAAGGCCGCCACGAAAAAGCAAAAGTATGAGAAGATTTCTGAGAAGAAAATGACGACATCAGTCGAGCACTTGTGCAAAGGATTCCCAGCTGAGTTCCCAATGTACTTGAGCTACACAAGAGGACTTCGTTTCGATGAATCTCCAGATTACATGTACTTGCGGCAACTGTTCAGAATTCTCTTCAGAACGCTCAATCATCAGTACGACTACACATTCGACTGGACAATGCTCAAACAAAAGGCTCAACAATCGCAATCCAGTGGCGTCCCCGGAACCAACACTACTACACAGGGAGCTACCGTTCCATCAGCTGGAGTTCCAGCTGGAGTTGCACCAGGAGGAACTACTCCACAGTAA
ExperimentLaboratoryCT
Date01 Nov 2010 00:00:00
TreatmentRNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneC03C10.1Inferred_automaticallyRNAi_primary
GeneWBGene00002202Inferred_automaticallyRNAi_primary
TranscriptC03C10.1.3Inferred_automaticallyRNAi_primary
C03C10.1.1Inferred_automaticallyRNAi_primary
C03C10.1.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00037838
PhenotypeWBPhenotype:0000022RemarkAt the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile.
EQ_annotationsLife_stageWBls:0000041PATO:0000460
WBPhenotype:0000164RemarkAt the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile.
EQ_annotationsLife_stageWBls:0000041PATO:0000460
WBPhenotype:0000688RemarkAt the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile.
EQ_annotationsLife_stageWBls:0000041PATO:0000460
WBPhenotype:0000697RemarkAt the gross anatomical level, kin-19(RNAi) resulted in adult animals that were thin and elongated in shape, had protruding vulva (Pvl), and were sterile.
EQ_annotationsLife_stageWBls:0000041PATO:0000460
Remark(Data not shown) kin-19 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.
MethodRNAi