WormBase Tree Display for RNAi: WBRNAi00101799
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| WBRNAi00101799 | Homol | Homol_homol | C10G8:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | mv_C10G8.6 | |||
| Experiment | Laboratory | LW | |||
| Date | 04 May 2009 00:00:00 | ||||
| Genotype | pop-1(q645) I; rrf-3(pk1426) II; ccIs4438 (intrinsic CC(CoelomoCyte)::gfp) III | ||||
| Treatment | To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating. | ||||
| Temperature | 25 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | C10G8.6 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00000455 | Inferred_automatically | RNAi_primary | ||
| Transcript | C10G8.6.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00033137 | ||||
| Phenotype | WBPhenotype:0000095 | Remark | Postembryonic ceh-34 RNAi in the pop-1(q645) mutant background results in fewer coelomocytes derived from the M-lineage (or none) as determined by CC::GFP, opposite the extra coelomocytes phenotype of pop-1(q645) mutants, suggesting that ceh-34(RNAi) is epistatic to pop-1(q645) | ||
| Penetrance | Complete | ||||
| WBPhenotype:0000961 | Remark | Postembryonic ceh-34 RNAi in the pop-1(q645) mutant background results in fewer coelomocytes derived from the M-lineage (or none) as determined by CC::GFP, opposite the extra coelomocytes phenotype of pop-1(q645) mutants, suggesting that ceh-34(RNAi) is epistatic to pop-1(q645) | |||
| Penetrance | Complete | ||||
| WBPhenotype:0001912 | Remark | Postembryonic ceh-34 RNAi in the pop-1(q645) mutant background results in fewer coelomocytes derived from the M-lineage (or none) as determined by CC::GFP, opposite the extra coelomocytes phenotype of pop-1(q645) mutants, suggesting that ceh-34(RNAi) is epistatic to pop-1(q645) | |||
| Penetrance | Complete | ||||
| Remark | (Table 2) ceh-34 RNAi-P (postembryonic RNAi) | ||||
| Method | RNAi |
