WormBase Tree Display for RNAi: WBRNAi00101803

WBRNAi00101803 Homol: Homol_homol: B0336:RNAi
  Sequence_info: PCR_product: sjj_B0336.1
  Experiment: Laboratory: LW
    Date: 04 May 2009 00:00:00
    Genotype: rrf-3(pk1426) II; ayIs6(hlh-8::gfp) X
    Treatment: To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: B0336.1a Inferred_automatically: RNAi_primary
      B0336.1b Inferred_automatically: RNAi_primary
    Gene: WBGene00006943 Inferred_automatically: RNAi_primary
    Transcript: B0336.1b.1 Inferred_automatically: RNAi_primary
      B0336.1a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00033137
  Phenotype: WBPhenotype:0000095 Remark: Postembryonic wrm-1(RNAi) resulted in animals with extra sex myoblasts (SMs) derived from the M-lineage (in 77.8% of animals), as determined by hlh-8::GFP
      Penetrance: Range: 78
    WBPhenotype:0000961 Remark: Postembryonic wrm-1(RNAi) resulted in animals with extra sex myoblasts (SMs) derived from the M-lineage (in 77.8% of animals), as determined by hlh-8::GFP
      Penetrance: Range: 78
    WBPhenotype:0002192 Remark: Postembryonic wrm-1(RNAi) resulted in animals with extra sex myoblasts (SMs) derived from the M-lineage (in 77.8% of animals), as determined by hlh-8::GFP
      Penetrance: Range: 78
  Remark: (Table 2) wrm-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi