WormBase Tree Display for RNAi: WBRNAi00101805

WBRNAi00101805 Homol: Homol_homol: T23D8:RNAi
  Sequence_info: PCR_product: sjj_T23D8.9
  Experiment: Laboratory: LW
    Date: 04 May 2009 00:00:00
    Genotype: rrf-3(pk1426) II; ayIs6(hlh-8::gfp) X
    Treatment: To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: T23D8.9a Inferred_automatically: RNAi_primary
      T23D8.9c Inferred_automatically: RNAi_primary
      T23D8.9b Inferred_automatically: RNAi_primary
    Gene: WBGene00006379 Inferred_automatically: RNAi_primary
    Transcript: T23D8.9b.1 Inferred_automatically: RNAi_primary
      T23D8.9c.1 Inferred_automatically: RNAi_primary
      T23D8.9a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00033137
  Phenotype: WBPhenotype:0000095 Remark: Postembryonic sys-1(RNAi) resulted in animals with extra ventral sex myoblasts (SMs) derived from the M-lineage (in 45.8% of animals), as determined by hlh-8::GFP. Authors argue that the extra SMs are likely the result of M.vlpp and/or M.vrpp cells undergoing a posterior-to-anterior fate transformation to that of sister cells M.vlpa and/or M.vrpa.
      Penetrance: Range: 46
    WBPhenotype:0000414 Remark: Postembryonic sys-1(RNAi) resulted in animals with extra ventral sex myoblasts (SMs) derived from the M-lineage (in 45.8% of animals), as determined by hlh-8::GFP. Authors argue that the extra SMs are likely the result of M.vlpp and/or M.vrpp cells undergoing a posterior-to-anterior fate transformation to that of sister cells M.vlpa and/or M.vrpa.
      Penetrance: Range: 46
    WBPhenotype:0000961 Remark: Postembryonic sys-1(RNAi) resulted in animals with extra ventral sex myoblasts (SMs) derived from the M-lineage (in 45.8% of animals), as determined by hlh-8::GFP. Authors argue that the extra SMs are likely the result of M.vlpp and/or M.vrpp cells undergoing a posterior-to-anterior fate transformation to that of sister cells M.vlpa and/or M.vrpa.
      Penetrance: Range: 46
    WBPhenotype:0002192 Remark: Postembryonic sys-1(RNAi) resulted in animals with extra ventral sex myoblasts (SMs) derived from the M-lineage (in 45.8% of animals), as determined by hlh-8::GFP. Authors argue that the extra SMs are likely the result of M.vlpp and/or M.vrpp cells undergoing a posterior-to-anterior fate transformation to that of sister cells M.vlpa and/or M.vrpa.
      Penetrance: Range: 46
  Remark: (Table 2) sys-1 RNAi-P (postembryonic RNAi)
  Method: RNAi