WormBase Tree Display for RNAi: WBRNAi00103853

WBRNAi00103853 Homol: Homol_homol: Y38A8:RNAi
  Sequence_info: DNA_text: ATGAGTGATTCAACTCAAATGGAGGCCAATGCTGCTATTAATGAAATTAAAAAACGAACGAGAAAGCTAGACCAATCGCTGCAAATGAGGTTTTGCGCTGATCAGTTTTTCATCGGCAATCATTGCACCAAACTGGCATCCGGAAAGTCAATAATTATCTCGCAGAATTCCAAGAATCGGATATGCCTTCGCTTTTTCATGGCAGCGGATCCTTTGGTGGGCTACACTGGACGAGATTTTGGAATCGCTTTCAATCAGATAGACCACATTTCGCTGAAAGATGAGCAACAAGAGAATCCCGCTGTACTCATTTGCACTTTGAACCTCTCAAGCTACACAAAAATGTGTAAGCTCCAAACCGGCTTGAAGGATGTCGTCGAGAAACCATTTCTGTATAACAAATCTCTTGCACGGAATTTGACATTCATACTTAAACCGTGGAACGATGACCCTGACACGTTTGTGAAGATCTCATACAATGATGAACACCGAGAGTATGTGCACAGCTATGATTACGATGTGGCCAAGTCCTTAATGTTCAAAGAG
      GATTTTCCAAACTTTGACTGGTCAAAGTTCTTCCCGGAGGCCAATAAAATGTGTGACTTGATGCGAGACAAAGTTTACAATTTGATTCTTCAACAAGCGGATAAACCTGCGAGAAGCCGTCTCGCCAAGTTCGAAAGGGAGAACAAATGTGGATTGTCGCGTGAAGGAGCTCTTAGGAAAGCACGTCGTCATAGTGCAGTGAATGAACGTCGTACGAAACGGCACAGAGACTATTATGCACGTCACTATTCCTTGAGCCCGCCACACCGAAATGTGATGAACGATGATCCGACGTTCATGAATCCACGTGGATTGGCGGAAATGCCAATAACTCGACTTGTTCGTCGCCTCAGAATTCCGGAGGATAACTTCCCAATCGCTTACTAG
  Experiment: Genotype: HMR-1::GFP; VAB-10ABD::mCherry
    Treatment: Injected worms were allowed to recover on NGM plates overnight at 20 degrees Celsius and then transferred to fresh plates. F1 gravid adults were cut and the released F2 embryos were analyzed.
    Temperature: 20
    Delivered_by: Injection
  Inhibits: Predicted_gene: Y38A8.3c Inferred_automatically: RNAi_primary
      Y38A8.3a Inferred_automatically: RNAi_primary
      Y38A8.3b Inferred_automatically: RNAi_primary
    Gene: WBGene00006737 Inferred_automatically: RNAi_primary
    Transcript: Y38A8.3c.1 Inferred_automatically: RNAi_primary
      Y38A8.3b.1 Inferred_automatically: RNAi_primary
      Y38A8.3a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00048594
  Phenotype: WBPhenotype:0000679 Remark: Embryos subjected to ulp-2 RNAi exhibited discontinuous HMR-1::GFP foci and collapsed actin bundles (Figures 7C-7E). In WT embryos, actin bundles are arranged in parallel bands, and HMR-1::GFP is localized at the base of the actin bundle anchoring it to the membrane at AJs. In ulp-2(RNAi) embryos, loss of HMR-1 at the apical junction is accompanied by collapse and random spacing of actin bundles.
      EQ_annotations: Life_stage: WBls:0000003 PATO:0000460
        GO_term: GO:0051017 PATO:0000460
    WBPhenotype:0002124 Remark: Embryos subjected to ulp-2 RNAi exhibited discontinuous HMR-1::GFP foci and collapsed actin bundles (Figures 7C-7E). In WT embryos, actin bundles are arranged in parallel bands, and HMR-1::GFP is localized at the base of the actin bundle anchoring it to the membrane at AJs. In ulp-2(RNAi) embryos, loss of HMR-1 at the apical junction is accompanied by collapse and random spacing of actin bundles.
      EQ_annotations: Life_stage: WBls:0000003 PATO:0000460
        GO_term: GO:0051017 PATO:0000460
  Remark: (Figure 7C-E) ulp-2 RNAi against exons 1-4 and 15
  Method: RNAi