WormBase Tree Display for RNAi: WBRNAi00106278

WBRNAi00106278 Homol: Homol_homol: C47E8:RNAi
  Sequence_info: PCR_product: sjj_C47E8.5
  Experiment: Genotype: unc-45(e286)
    Treatment: Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15°C) were treated with RNAi for different molecular chaperones and monitored for motility defects.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: C47E8.11 Inferred_automatically: RNAi_primary
      C47E8.5 Inferred_automatically: RNAi_primary
    Gene: WBGene00044180 Inferred_automatically: RNAi_primary
      WBGene00000915 Inferred_automatically: RNAi_primary
    Transcript: C47E8.5.1 Inferred_automatically: RNAi_primary
      C47E8.11.1 Inferred_automatically: RNAi_primary
      C47E8.5.2 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00046852
  Phenotype: WBPhenotype:0000059 Remark: "RNAi knockdown of chaperones was next performed with unc-45(ts) mutant strains under permissive conditions. Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15C) were treated with RNAi for different molecular chaperones and monitored for motility defects. We first sought chaperones that cause unc-45(e286)-specific phenotypes at permissive conditions when depleted (Figure 2A). Of the 96 RNAi constructs tested, five genes induced loss of coordination and/or an egg-laying defect, resulting in reduced motility in more than 70% of the unc-45(e286) mutant animals. As noted above, down-regulation of unc-45 or unc-23 also induced a loss of coordination in wild-type animals (Table 1); these were discarded from the candidate gene list (Figure 2A). The remaining chaperones identified in the screen are CeHsp90 (daf-21), CeHop (sti-1) and CeAha1 (C01G10.8) (Figures 2B,C)." "RNAi treatment for CeHsp90, encoding a known myosin chaperone, resulted in a strong larval arrest phenotype in both wild-type and unc45(e286) animals but induced a strong motility defect only in unc-45(e286) animals (76.4 8.3%, as compared to 12.1 4% motile animals)."
    WBPhenotype:0000644 Remark: "RNAi knockdown of chaperones was next performed with unc-45(ts) mutant strains under permissive conditions. Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15C) were treated with RNAi for different molecular chaperones and monitored for motility defects. We first sought chaperones that cause unc-45(e286)-specific phenotypes at permissive conditions when depleted (Figure 2A). Of the 96 RNAi constructs tested, five genes induced loss of coordination and/or an egg-laying defect, resulting in reduced motility in more than 70% of the unc-45(e286) mutant animals. As noted above, down-regulation of unc-45 or unc-23 also induced a loss of coordination in wild-type animals (Table 1); these were discarded from the candidate gene list (Figure 2A). The remaining chaperones identified in the screen are CeHsp90 (daf-21), CeHop (sti-1) and CeAha1 (C01G10.8) (Figures 2B,C)." "RNAi treatment for CeHsp90, encoding a known myosin chaperone, resulted in a strong larval arrest phenotype in both wild-type and unc45(e286) animals but induced a strong motility defect only in unc-45(e286) animals (76.4 8.3%, as compared to 12.1 4% motile animals)."
  Remark: (Figure 2) daf-21/CeHsp90 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
  Method: RNAi