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WormBase Tree Display for RNAi: WBRNAi00107879

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Name Class

WBRNAi00107879HomolHomol_homolCHROMOSOME_II:RNAi
Sequence_infoPCR_productsjj_ZK669.1
ExperimentTreatment"L4 hermaphrodites were injected with dsRNAs prepared by using the oligonucleotides listed in the table above to PCR-amplify regions from the indicated templates. Injected worms were incubated at 20 degrees Celsius for 20-28 h before dissection and imaging of their embryos (cyk-4(RNAi), 23-27 h; cdc-42, rac-2, ced-10, and ect-2(RNAi); 20-24 h; rho-1(RNAi), 24-28 h). For embryonic lethality tests, worms were singled 23-27 h post injection and were allowed to lay embryos for 24 h at 20C. Adult worms were removed and hatched larvae and unhatched embryos were counted 24 h later."
Delivered_byInjection
InhibitsPredicted_geneZK669.1eInferred_automaticallyRNAi_primary
ZK669.1cInferred_automaticallyRNAi_primary
ZK669.1bInferred_automaticallyRNAi_primary
ZK669.1dInferred_automaticallyRNAi_primary
ZK669.1aInferred_automaticallyRNAi_primary
GeneWBGene00014051Inferred_automaticallyRNAi_primary
TranscriptZK669.1d.1Inferred_automaticallyRNAi_primary
ZK669.1c.1Inferred_automaticallyRNAi_primary
ZK669.1d.2Inferred_automaticallyRNAi_primary
ZK669.1a.1Inferred_automaticallyRNAi_primary
ZK669.1a.2Inferred_automaticallyRNAi_primary
ZK669.1e.1Inferred_automaticallyRNAi_primary
ZK669.1b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00044595
Phenotype_not_observedWBPhenotype:0000886RemarkAuthors report a wild type phenotype (Table S1)
Remark(Table S1) tag-341 RNAi
MethodRNAi