WormBase Tree Display for Strain: WBStrain00005267
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| WBStrain00005267 | Status | Live | ||
|---|---|---|---|---|
| Genotype | slt-1(eh15) X. | |||
| Public_name | CX5000 | |||
| Contains | Gene | WBGene00004854 | ||
| Variation | WBVar00145128 | |||
| Properties | Outcrossed | x2 | ||
| CGC_received | 26 Nov 2001 00:00:00 | |||
| Location | CGC | |||
| Remark | slt-1 mutants have no dissecting-scope phenotype. They have a 40% penetrant defect in the ventral guidance of the AVM neuron scored with mec-4::GFP, a mild defect in CAN cell migration that is enhanced by a ceh-23::GFP transgene, and a mild defect in midline crossing by PVQ neurons scorable with sra-6::GFP. slt-1(eh15) is a complex rearrangement that duplicates the endogenous slt-1 gene, but disrupts both duplicated copies. The two copies are linked on X but the exact distance between them is not known. The duplication probably extends >13 kb based on Southern blotting. Deletion breakpoints for the first copy of slt-1 are as follows: nucleotides 26219 to 28163 and 28197 to 28294 in cosmid C26G2 are deleted. The second copy of slt-1 contains the following structure: nucleotides 28197 to 28294 in C26G2 are deleted, followed by a duplication of nucleotides 28300 to 28396 in C26G2 that begins 5 nucleotides after the deletion. Both copies of slt-1 are mutant, as confirmed by both DNA sequence and RT-PCR analysis of slt-1 mRNA. Scoring for homozygosity of the slt-1 allele by PCR is difficult because of the two copies of the gene and because the small deletion and the small duplication of the second copy of slt-1 are the same size. The mutant can be followed indirectly by X linkage (very closely linked to unc-3). It may be possible to make a specific primer within the duplicated region that detects a unique band in the slt-1 mutant. | Inferred_automatically | From CGC strain data | |
| Made_by: David Hughes/Joe Hao | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
