WormBase Tree Display for Strain: WBStrain00024074
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| WBStrain00024074 | Status | Live | ||
|---|---|---|---|---|
| Genotype | pha-1(e2123) III; him-5(e1490) V; rnyEx109. | |||
| Public_name | KWN190 | |||
| Contains | Gene | WBGene00001864 | ||
| WBGene00004010 | ||||
| Variation | WBVar00144039 | |||
| WBVar00144583 | ||||
| Transgene | WBTransgene00016244 | |||
| Properties | CGC_received | 22 Apr 2014 00:00:00 | ||
| Location | CGC | |||
| Made_by | WBPerson2233 | |||
| Remark | rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613. | Inferred_automatically | From CGC strain data | |
| WBStrain provided so WBPaper00061870 paper added based on AFP_Strain data. | Curator_confirmed | WBPerson1983 | ||
| Reference | WBPaper00061870 | |||
| Species | Caenorhabditis elegans |
