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WormBase Tree Display for Strain: WBStrain00037202

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Name Class

WBStrain00037202StatusLive
Genotypenstp-3(ok2873) V/nT1 [qIs51] (IV;V).
Public_nameVC2250
ContainsGeneWBGene00022577
VariationWBVar00093968
RearrangementnT1
nT1[qIs51]
TransgeneWBTransgene00001903
PropertiesOutcrossedx1
MutagenEMS
CGC_received24 Sep 2008 00:00:00
LocationCGC
Made_byWBPerson20179
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
ZC250.3. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2873 homozygotes (late larval arrest or sterile adult). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCCTTATCCCAAATTCCCTT. External right primer: CGTTTCATTGGGATACCTGG. Internal left primer: TTTTTGCAAATTTCCATCCG. Internal right primer: AATCTTGGCATCCACCTCAC. Internal WT amplicon: 1225 bp. Deletion size: 429 bp. Deletion left flank: TTAATAGGAAGCTGAGAATGTCAATTTTTG. Deletion right flank: AACTGATGAAAAATGGAAGAATTTAGGAAA.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans