WormBase Tree Display for Strain: WBStrain00051653
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WBStrain00051653 | Status | Live |
---|---|---|
Genotype | unc-119(ox819) F53A2.9(oxTi1127) III. | |
Public_name | EG9881 | |
Contains (2) | ||
Properties (2) | ||
Location | CGC | |
Made_by | WBPerson22962 | |
Remark | oxTi1127 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Inserted into F53A2.9. High Cas9 activity. Integrated Cas9 transgene linked to unc-119(ox819). Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https: | |
Species | Caenorhabditis elegans |