WormBase Tree Display for Transgene: WBTransgene00000613
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WBTransgene00000613 | Public_name | huIs1 | ||||||
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Summary | [hs::delNTbar-1] | |||||||
Construction | Construct | WBCnstr00000611 | ||||||
Construction_summary | huIs1 indicates the integrated array containing the hs delNTbar-1 construct, pHCK19 (50 ng/L), and a dpy-20(+) marker plasmid, pMH86 (100 ng/L). pHCK19 contains sequences downstream from the natural NcoI site in the 5 end of the bar-1 cDNA driven from the heat-shock promoter in plasmid pPD49.78. | |||||||
Genetic_information | Integrated | |||||||
Phenotype | WBPhenotype:0000218 | Paper_evidence | WBPaper00026863 | |||||
WBPaper00005277 | ||||||||
Curator_confirmed | WBPerson712 | |||||||
WBPerson2987 | ||||||||
Remark | On average 4.53 VCPs (n=15 heat shocked animals) are induced to adopt a vulval cell fate, which is increased compared to wild type (3/6 VPCs induced, n=29). VPCs and fate adoption were scored by Nomarski optics. | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
"... we expressed delNTBAR-1 from the C. elegans heat-shock promoter (hs::delNTbar-1) to produce higher levels of the truncated protein. When hs::delNTbar-1 was introduced into wild-type animals on either an extrachromosomal (data not shown) or integrated array (huIs1) and subjected to heat shocks during the L2 and L3 stages, the majority of animals displayed an Overinduced phenotype (Table 1). Invaginations due to ectopic division of P3.p, P4.p, and P8.p were often seen (Fig 1)." | Paper_evidence | WBPaper00005277 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | hs::delNTbar-1 | Paper_evidence | WBPaper00005277 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | hsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290. | Paper_evidence | WBPaper00026863 | ||||
Curator_confirmed | WBPerson712 | |||||||
Animals exposed to heat shock during the L2 and L3 larval stages. Heat shock at L1 or early L2 was not effective at inducing the phenotype, but heat shock at the L2/L3 molt was very effective. | Paper_evidence | WBPaper00005277 | ||||||
Curator_confirmed | WBPerson2987 | |||||||
WBPhenotype:0000700 | Paper_evidence | WBPaper00026863 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | 66% animals display one or more ectopic pseudovulvae after heat shock treatment (n=76). | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | hsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290. | Paper_evidence | WBPaper00026863 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0000698 | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | 0% heat shocked animals (n=15) are aberrant in induction of VPCs as scored by Nomarski optics. | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Variation_effect | Hypermorph_gain_of_function | Paper_evidence | WBPaper00026863 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | hsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290. | Paper_evidence | WBPaper00026863 | ||||
Curator_confirmed | WBPerson712 | |||||||
Used_for | Interactor | WBInteraction000535550 | ||||||
WBInteraction000549461 | ||||||||
Reference | WBPaper00005277 | |||||||
WBPaper00026863 | ||||||||
WBPaper00037609 | ||||||||
WBPaper00042342 | ||||||||
WBPaper00046887 | ||||||||
Species | Caenorhabditis elegans |