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WormBase Tree Display for Transgene: WBTransgene00000613

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Name Class

WBTransgene00000613Public_namehuIs1
Summary[hs::delNTbar-1]
ConstructionConstructWBCnstr00000611
Construction_summaryhuIs1 indicates the integrated array containing the hs delNTbar-1 construct, pHCK19 (50 ng/L), and a dpy-20(+) marker plasmid, pMH86 (100 ng/L). pHCK19 contains sequences downstream from the natural NcoI site in the 5 end of the bar-1 cDNA driven from the heat-shock promoter in plasmid pPD49.78.
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000218Paper_evidenceWBPaper00026863
WBPaper00005277
Curator_confirmedWBPerson712
WBPerson2987
RemarkOn average 4.53 VCPs (n=15 heat shocked animals) are induced to adopt a vulval cell fate, which is increased compared to wild type (3/6 VPCs induced, n=29). VPCs and fate adoption were scored by Nomarski optics.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
"... we expressed delNTBAR-1 from the C. elegans heat-shock promoter (hs::delNTbar-1) to produce higher levels of the truncated protein. When hs::delNTbar-1 was introduced into wild-type animals on either an extrachromosomal (data not shown) or integrated array (huIs1) and subjected to heat shocks during the L2 and L3 stages, the majority of animals displayed an Overinduced phenotype (Table 1). Invaginations due to ectopic division of P3.p, P4.p, and P8.p were often seen (Fig 1)."Paper_evidenceWBPaper00005277
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00000238Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Caused_by_otherhs::delNTbar-1Paper_evidenceWBPaper00005277
Curator_confirmedWBPerson2987
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_assayTreatmenthsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Animals exposed to heat shock during the L2 and L3 larval stages. Heat shock at L1 or early L2 was not effective at inducing the phenotype, but heat shock at the L2/L3 molt was very effective.Paper_evidenceWBPaper00005277
Curator_confirmedWBPerson2987
WBPhenotype:0000700Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Remark66% animals display one or more ectopic pseudovulvae after heat shock treatment (n=76).Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000238Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_assayTreatmenthsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000698Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Remark0% heat shocked animals (n=15) are aberrant in induction of VPCs as scored by Nomarski optics.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000238Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Variation_effectHypermorph_gain_of_functionPaper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Phenotype_assayTreatmenthsIs1 contains gain-of-function bar-1 under control of a heat shock promoter. Heat shock treatment followed the protocol published in Gleason et al. 2002 Genes Dev. 16:1281-1290.Paper_evidenceWBPaper00026863
Curator_confirmedWBPerson712
Used_forInteractorWBInteraction000535550
WBInteraction000549461
ReferenceWBPaper00005277
WBPaper00026863
WBPaper00037609
WBPaper00042342
WBPaper00046887
SpeciesCaenorhabditis elegans