WormBase Tree Display for Transgene: WBTransgene00000726
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| WBTransgene00000726 | Public_name | juIs109 | |
|---|---|---|---|
| Summary | [efn-4::GFP + lin-15(+)] | ||
| Synonym | [efn-4::gfp] | ||
| Construction | Construct | WBCnstr00000722 | |
| Integration_method | X-ray | ||
| Construction_summary | Clone = pCZ147. A 16.1 HindIII-EagI genomic DNA fragment was cloned from cosmid F56A11 into pSL1190, creating pCZ148. This clone contains 5.3 kb genomic sequence 5' to the EFN-4 ATG. An EFN-4::GFP reporter was constructed by insertion of the GFP intron cassette from vector pPD121.89 into the Sac II site of the rescuing genomic clone pCZ148, to create the EFN-4::GFP clone pCZ147. This clone is predicted to encode EFN-4 with GFP inserted in-frame close to the N terminus of the mature protein, after residue 33. juIs109 is the chromosomal integrant of juEx210. juEx210) formed by co-injection of pCZ147 and the lin-15(+) plasmid pLin-15EK into lin-15(n765) animals. --precise ends. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2300 | |
| Expr12755 | |||
| Expr12914 | |||
| Associated_with | Strain | WBStrain00005365 | |
| Reference | WBPaper00005631 | ||
| WBPaper00029396 | |||
| WBPaper00048941 | |||
| WBPaper00049274 | |||
| WBPaper00049404 | |||
| Species | Caenorhabditis elegans |
