WormBase Tree Display for Transgene: WBTransgene00000757
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| WBTransgene00000757 | Public_name | kcIs6 | |
|---|---|---|---|
| Summary | [ifb-2p::IFB-2::CFP] | ||
| Construction | Construct | WBCnstr00000753 | |
| Integration_method | UV | ||
| Construction_summary | A plasmid construct encoding IFB-2::CFP was prepared by first amplifying the 1,994 bp ifb-2 promoter fragment from genomic DNA with the help of amplimers 05-143 (5' -CCG CCG AAG CTT CCA TAG GGA AAT CGT GTT ATC-3') and 05-144 (5' -CCG CCG CTG CAG GAT GAA GTC GCT AAA ATT TTG-3'). The HindIII/PstI-cleaved fragment was inserted into the HindIII/PstI sites of the cfp-containing vector pVH10.10, thereby generating plasmid pPifb-2:cfp. For cloning of the 1,646 bp ifb-2 cDNA fragment, ifb-2 cDNA was amplified by PCR using primers 05-145 (5' -CCA CCA CTG CAG ATG TCG GCG GTT AGT TAT TCG-3') and 05-146 (5' -TTA TTA CTG CAG GCA GCG ACC GTC GTC TGG ATG-3'). The PstI-digested product was finally inserted into pPifb-2:cfp to produce pifb-2::cfp. Correct cloning was confirmed by DNA sequencing. | ||
| Genetic_information | Integrated | ||
| Map | IV | ||
| Used_for | Expr_pattern | Expr8186 | |
| Expr14279 | |||
| Expr15262 | |||
| Associated_with | Strain | WBStrain00042346 | |
| Reference (14) | |||
| Species | Caenorhabditis elegans |
