WormBase Tree Display for Transgene: WBTransgene00000802

WBTransgene00000802 Public_name: kuIs36
  Summary: [unc-119(+) egl-26::GFP]
  Synonym: [Pegl-26::gfp; unc-119(+)]
  Construction: Construct: WBCnstr00000797
  Construction_summary: To make the GFP translational fusion, the region between the SacI site in the gene and the last codon was amplified by using primers that introduce SalI and BamHI sites on the 5' and 3' ends, respectively, and cloned into the SalI and BamHI sites of pPD95.77. The remaining egl-26 coding and regulatory regions were added by digesting with SalI and SacI and ligating to the 5.7-kbp SalISacI fragment from pWH11. The ApaIBamHI fragment was exchanged between pWH15 and pPD95.69 to add a nuclear localization signal, creating pWH16. To produce pWH22, the GFP transcriptional fusion, the egl-26 coding region was removed from pWH16 by replacing the NsiIBamHI fragment with an amplified fragment containing the region between the promoter NsiI site and an engineered BamHI site after the start codon.
    transcriptional fusion.
  Genetic_information: Integrated
    Map: II
  Used_for: Expr_pattern: Expr1807
      Expr12304
      Marker113
    Marker_for: Y rectal cell Paper_evidence: WBPaper00031556
    Interactor: WBInteraction000502426
      WBInteraction000502889
      WBInteraction000503843
      WBInteraction000505039
  Associated_with: Strain: WBStrain00026518
  Reference (12)
  Species: Caenorhabditis elegans