WormBase Tree Display for Transgene: WBTransgene00000807
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| WBTransgene00000807 | Public_name | kuIs55 | |
|---|---|---|---|
| Summary | [lon-3::GFP + unc-119(+)] | ||
| Synonym | [lon-3::gfp] | ||
| Construction | Construct | WBCnstr00000802 | |
| Construction_summary | translational fusion construct pYSL3G3. The translational reporter gene pYSL3G3 had the complete 5 and coding sequences present in pYSL3S2, followed by a GFP sequence, the last eight codons repeated of the lon-3 coding sequence, the native lon-3 stop codon, and the complete 3 sequence of pYSL3S2. To generate this plasmid, PCR with primer sequences, TGGTGGGC-GGCGCC (NarI)-GTAAGCACAAGAGAGTCTAC (the end of the lon-3 coding sequence)-ATGAGTAAAGGAGAAGAACTTTTCAC (the beginning of the GFP sequence) and TGGTGGGC-GGCGCC (NarI)-GATCCCCCGGGCATTTGTATAG (the end of the GFP sequence), was used to generate a fragment that had the GFP sequence connected to the last part, from the NarI site to the end, of the lon-3 coding sequence. This fragment flanked by NarI sites was cloned into the unique NarI site of pYSL3S2. --precise ends. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2362 | |
| Interactor | WBInteraction000501829 | ||
| WBInteraction000501830 | |||
| Associated_with | Strain | WBStrain00026524 | |
| Reference | WBPaper00005680 | ||
| WBPaper00046170 | |||
| WBPaper00059347 | |||
| Species | Caenorhabditis elegans |
