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WormBase Tree Display for Transgene: WBTransgene00001013

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Name Class

WBTransgene00001013Public_namempIs1
Summary[mig-10b::gfp; dpy-20(+)]
ConstructionConstructWBCnstr00001004
Integration_methodGamma_irradiation
Construction_summary[mig-10b::gfp] transcriptional GFP fusion construct. Four kilobases of sequence upstream of each translational start site was fused to GFP to produce the mig-10b::gfp transcriptional fusion construct. An integrated transgene, mpIs1, was created by injection of dpy-20(e1282ts) animals with 100 ug/ml dpy-20(+) DNA and 50 ug/ml of mig-10b::gfp. One of two similar resulting extrachromosomal array strains (mpEx2) was integrated. One hundred L4 dpy-20(e1282ts); mpEx2 animals were irradiated with a gamma source (4000 Rads).
Genetic_informationIntegrated
Used_forExpr_patternExpr3865
Associated_withStrainWBStrain00033874
ReferenceWBPaper00027185
WBPaper00035213
SpeciesCaenorhabditis elegans