WormBase Tree Display for Transgene: WBTransgene00004863
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| WBTransgene00004863 | Public_name | vaIs3 | |
|---|---|---|---|
| Summary | [cca-1::GFP] | ||
| Construction | Construct | WBCnstr00004844 | |
| Construction_summary | The construct fused an 8074 base pair (bp) fragment of the cca-1 gene comprising 5.4 kb of sequence upstream of the putative cca-1 initiation codon and three coding exons to GFP. The cca-1 fragment was amplified by PCR using the primers cca-11 (5'-TGATGATAGGACGCTGGTCA-3') and cca-12 (5'-TGTTCCGACAGCTGCAGAGT-3'). The PCR product was digested with PvuII and PstI and cloned into pBluescript SK. The GFP gene and unc-54 3' UTR regions of pPD95.69 were isolated by digestion with PstI and EagI and the resulting 1.9 kb fragment was inserted into pBluescript, 3' to the cca-1 sequences, to generate an in-frame cca-1::GFP fusion (pcca-1::GFP). --precise ends. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr4350 | |
| Associated_with | Strain | WBStrain00035024 | |
| Reference | WBPaper00025210 | ||
| Species | Caenorhabditis elegans |
