WormBase Tree Display for Transgene: WBTransgene00005248
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WBTransgene00005248 | Public_name | zxIs3 | ||||||
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Summary | [unc-47p::ChR2(H134R)::YFP + lin-15(+)] | |||||||
Synonym | [Punc-47::chop-2(H134R)-yfp; lin-15(+)] | |||||||
Construction | Construct | WBCnstr00005225 | ||||||
Construction_summary | 80 ng/ul unc-47p::ChR2(H134R)::YFP | |||||||
Genetic_information | Integrated | |||||||
Phenotype | WBPhenotype:0000644 | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals became almost completely paralyzed after illumination (photoactivation of ChR2-YFP in GABAergic neurons). Paralysis was reduced within 3 sec of sustained illumination, and animals resumed movement, though they were uncoordinated and slow. | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Human channelrhodopsin-2 (H134R) | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. | Paper_evidence | WBPaper00032190 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001478 | Curator_confirmed | WBPerson712 | ||||||
Remark | "...expression of the channelrhodopsin-2 (ChR2) gain-of-function mutant H134R in GABAergic neurons results in about 4 % of elongation, compared to about 3 % in ChrimsonSA expressing animals (Fig. 1C+D, Liewald et al., 2008). However, in contrast to ChR2, ChrimsonSA expressing animals do not show a decline of body length during stimulation. | Curator_confirmed | WBPerson712 | |||||
Phenotype_assay | Treatment | Animals were treated with the chromophore all-trans-retinal (ATR), and a 10 s light stimulus. | Curator_confirmed | WBPerson712 | ||||
WBPhenotype:0001802 | Paper_evidence | WBPaper00032190 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Animals became elongated, 104.1% of the initial length within ~350 ms of illumination (photoactivation of ChR2-YFP in GABAergic neurons). This body elongation was reversible by darkness, but did not attenuate under constant illumination. | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Human channelrhodopsin-2 (H134R) | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. | Paper_evidence | WBPaper00032190 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0001316 | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Exogenous neurotransmitter evoked currents were no different from wild-type. | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Human channelrhodopsin-2 (H134R) | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. | Paper_evidence | WBPaper00032190 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001318 | Paper_evidence | WBPaper00032190 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | The frequency and amplitude of endogenous miniature postsynaptic currents of photostimulated ChR2-YFP containing GABAergic neurons were comparable to wild-type. | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Human channelrhodopsin-2 (H134R) | Paper_evidence | WBPaper00032190 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Treatment | ChR2-YFP was activated in animals swimming in liquid, or on solid substrates, by applying 450-490 nm light. The chromophore essential fro ChR2 function was supplied by growing transgenic animals on medium containing all-trans retinal. | Paper_evidence | WBPaper00032190 | ||||
Curator_confirmed | WBPerson712 | |||||||
Associated_with | Strain | WBStrain00040910 | ||||||
Reference (13) | ||||||||
Species | Caenorhabditis elegans |