WormBase Tree Display for Transgene: WBTransgene00006487
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| WBTransgene00006487 | Public_name | fdEx7 | |
|---|---|---|---|
| Summary | [xnp-1::GFP; pRF4] | ||
| Synonym | Expr3034_Ex | ||
| Construction | Construct | WBCnstr00006334 | |
| Coinjection | WBCnstr00004720 | ||
| Construction_summary | To make an xnp-1::GFP transcriptional fusion construct, 3500 bp of sequence upstream of the xnp-1 start codon was amplified and cloned in frame into vector pPD95.69, which contains a GFP/nuclear localization sequence (NLS), as well as into vector pPD95.77, which contains only a GFP cassette (both kindly provided by A. Fire). Primer promf1 [5-AGCGTCGACCATTTCATTGAACACAT-3] contains a SalI site at the 5-prime end. Primer promr1 [5-ATAGGATCCAACTCTCATTAGTTGACA-3] contains a BamHI site at the 5-prime end and was designed to encompass the xnp-1 start codon and preserve the GFP reading frame. PCR was done using purified T04D1 cosmid DNA. The resulting product was digested with SalI and BamHI and ligated to the above vectors. Microinjections for xnp-1::GFP expression were carried out using between 100 and 200 ng/ul of purified plasmid DNA along with the rol-6 marker (pRF4). Several transmitting lines for each construct were obtained, all giving very similar expression patterns. One stable line containing the GFP::NLS sequence was used to generate the images. | ||
| Genetic_information | Extrachromosomal | ||
| Reference | WBPaper00005137 | ||
| WBPaper00024322 | |||
| Species | Caenorhabditis elegans |
