WormBase Tree Display for Transgene: WBTransgene00007117
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| WBTransgene00007117 | Public_name | WBPaper00036348Ex1 | |||||
|---|---|---|---|---|---|---|---|
| Summary | [rgef-1p::raga-1(DN)] | ||||||
| Construction | Construct | WBCnstr00006942 | |||||
| Construction_summary | This construct results in pan-neuronal (rgef-1) expressed dominant negative (DN) RAG protein, due to a T18N mutation resulting in constitutive GDP-binding. Transgenic worms bearing extra chromosomal arrays were generated with DNA at concentrations from 5-10 ng/l using standard techniques. Transgenic constructs were coinjected with either Pofm-1::GFP or Pmyo-3::mCherry as a marker. (Schreiber et al., 2010). | ||||||
| Genetic_information | Extrachromosomal | ||||||
| Phenotype | WBPhenotype:0000067 | Paper_evidence | WBPaper00003137 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Overexpression of HSB-1 resulted in lowered survival rates when animals were exposed to chemical stressors of sodium arsenite and mercuric chloride. In addition, animals exhibited a block in the activation of the stress response as measured by a reduction in heat-shock induced beta-galactosidase activity from a hsp-16::LacZ transgene. | Paper_evidence | WBPaper00003137 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_gene | WBGene00002002 | Paper_evidence | WBPaper00003137 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0001090 | Paper_evidence | WBPaper00003137 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | Overexpression of HSB-1 resulted in less fecundity, less survival and reduced hsp-16::LacZ regulated beta-galactosidase activity when animals were subjected to thermal stress. | Paper_evidence | WBPaper00003137 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Caused_by_gene | WBGene00002002 | Paper_evidence | WBPaper00003137 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00036348 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Remark | No substantial effects on lifespan | Paper_evidence | WBPaper00036348 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Caused_by_gene | WBGene00006414 | Paper_evidence | WBPaper00036348 | ||||
| Curator_confirmed | WBPerson2021 | ||||||
| Associated_with | Strain | WBStrain00042435 | |||||
| Reference | WBPaper00003137 | ||||||
| WBPaper00036348 | |||||||
| Species | Caenorhabditis elegans |
