WormBase Tree Display for Transgene: WBTransgene00007118
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WBTransgene00007118 | Public_name | WBPaper00036348Ex2 | |||||
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Summary | [rgef-1p::raga-1(DN)] | ||||||
Construction | Construct | WBCnstr00006943 | |||||
Construction_summary | This construct results in pan-neuronal (rgef-1) expressed dominant negative (DN) RAG protein, due to a T18N mutation resulting in constitutive GDP-binding. Transgenic worms bearing extra chromosomal arrays were generated with DNA at concentrations from 5-10 ng/l using standard techniques. Transgenic constructs were coinjected with either Pofm-1::GFP or Pmyo-3::mCherry as a marker. (Schreiber et al., 2010). | ||||||
Genetic_information | Extrachromosomal | ||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00036348 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Remark | No substantial effects on lifespan | Paper_evidence | WBPaper00036348 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Caused_by_gene | WBGene00006414 | Paper_evidence | WBPaper00036348 | ||||
Curator_confirmed | WBPerson2021 | ||||||
Associated_with | Strain | WBStrain00042436 | |||||
Reference | WBPaper00036348 | ||||||
Species | Caenorhabditis elegans |